Human G-CSF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK169-48, 70-EK169-96, EK169
- 分子靶点:CSF3
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:6.04pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:88%-116%
ELISA试剂盒详细信息
商品名 | 人粒细胞集落刺激因子酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | CSF3 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 6.04pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:7.4%-7.8%;板间变异系数:12.6%-8.7% |
回收率 | 88%-116% |
平均回收率 | 109% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人G-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的G-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中G-CSF的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CSF3 分子靶点信息概述
- 分子名:CSF3, colony stimulating factor 3
- 基因家族:Receptor ligands
- 别名:MGC45931
- 曾用名:GCSF; G-CSF; C17orf33
- 全称:granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)
CSF3 分子靶点综述
粒细胞集落刺激因子(G-CSF)是一种糖蛋白,可刺激骨髓产生粒细胞和干细胞,并把它们释放到血液中。从功能上来说,它是一种细胞因子和激素,是一种集落刺激因子,由内皮细胞、巨噬细胞和许多其它免疫细胞产生。G-CSF刺激中性粒细胞前体和成熟中性粒细胞的生存、增殖、分化及功能发挥。在肿瘤学和血液学上,重组G-CSF用于加速癌症患者化疗后引起的嗜中性白血球减少症的恢复。在白细胞去除前,G-CSF也可用来增加献血者血液中的造血干细胞数量,用于造血干细胞移植。
人 Human CSF3 分子靶点信息
- 分子名:CSF3, colony stimulating factor 3
- 别称:
- C17orf33
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
- CSF3OS
- filgrastim
- G-CSF
- GCSF
- granulocyte colony-stimulating factor
- granulocyte-colony stimulating factor
- lenograstim
- MGC45931
- pluripoietin
- 基因序列:NCBI_Gene: 1440
- 蛋白序列:UniProtKB: P09919
人 Human CSF3靶点分子功能(预测)
Enables cytokine activity and enzyme binding activity. Involved in several processes, including positive regulation of cytoskeleton organization; positive regulation of intracellular signal transduction; and positive regulation of protein phosphorylation. Predicted to be located in endocytic vesicle lumen; extracellular region; and lysosomal lumen. Predicted to be active in extracellular space. Implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Biomarker of COVID-19; bacterial pneumonia; and myeloid leukemia.
引用文献统计
该产品被引用的文献总数为:8
- Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma
影响因子:3.659刊物:CANCER BIOLOGY & THERAPY发表日期:2020/8/23 - Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis
影响因子:2.548刊物:Reproductive Sciences发表日期:2018/11/19 - G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma
影响因子:3.702刊物:Cancer Management and Research发表日期:2018/8/17 - Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling
影响因子:4刊物:Journal of Molecular Cell Biology发表日期:2020/4/2 - Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis
影响因子:4.631刊物:Journal of Inflammation Research发表日期:2022/9/17 - A gain-of-function variation in PLCG1 causes a new immune dysregulation disease
影响因子:14.2刊物:JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY发表日期:2023-07-06 - Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis
刊物:Reprod Sci. 2019 Aug;26(8):1130-1138. - G-Csf Associates with Neurogenesis and Predicts Prognosis and Sensitivity to Chemotherapy in Pancreatic Ductal Adenocarcinoma
刊物:Cancer management and research 10, 2767-2775 (2018)