Human BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1127-48, 70-EK1127-96, EK1127
- 分子靶点:BDNF
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本5μL
- 灵敏度:1.41pg/mL
- 检测范围:6.25-400pg/mL
- 回收率:82%-106%
ELISA试剂盒详细信息
商品名 | 人脑源性神经营养因子酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | BDNF |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本5μL |
灵敏度 | 1.41pg/mL |
线性范围 | 6.25-400pg/mL |
精密度 | 板内变异系数:5.6%-6.1%;板间变异系数:2.9%-7.9% |
回收率 | 82%-106% |
平均回收率 | 96% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
BDNF 分子靶点信息概述
- 分子名:BDNF, brain derived neurotrophic factor
- 基因家族:Neurotrophins
- 全称:neurotrophin
BDNF 分子靶点综述
脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。
人 Human BDNF 分子靶点信息
- 分子名:BDNF, brain derived neurotrophic factor
- 别称:
- abrineurin
- ANON2
- brain-derived neurotrophic factor
- BULN2
- MGC34632
- neurotrophin
- 基因序列:NCBI_Gene: 627
- 蛋白序列:UniProtKB: P23560
人 Human BDNF靶点分子功能(预测)
Predicted to enable growth factor activity and nerve growth factor receptor binding activity. Involved in nervous system development; positive regulation of nervous system development; and positive regulation of receptor binding activity. Located in mitochondrion and nuclear speck. Implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Biomarker of several diseases, including autoimmune disease (multiple); lung disease (multiple); mental depression (multiple); neurodegenerative disease (multiple); and open-angle glaucoma (multiple).
引用文献统计
该产品被引用的文献总数为:14
- Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy
影响因子:2.274刊物:NEUROSCIENCE LETTERS发表日期:2020/2/14 - A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism
影响因子:8.185刊物:Journal of Molecular Cell Biology发表日期:2022/2/10 - Peripheral Blood Circular RNAs as a Biomarker for Major Depressive Disorder and Prediction of Possible Pathways
影响因子:5.152刊物:Frontiers in Neuroscience发表日期:2022/3/31 - Analgesic Effects of Directed Repetitive Transcranial Magnetic Stimulation in Acute Neuropathic Pain After Spinal Cord Injury
影响因子:2.764刊物:PAIN MEDICINE发表日期:2019/11/13 - The effect of bilateral low-frequency rTMS over dorsolateral prefrontal cortex on serum brain-derived neurotropic factor and serotonin in patients with generalized anxiety disorder
影响因子:2.159刊物:NEUROSCIENCE LETTERS发表日期:2018/7/6 - High-efficiency brain-targeted intranasal delivery of BDNF mediated by engineered exosomes to promote remyelination
影响因子:7.59刊物:Biomaterials Science发表日期:2022/8/10 - Correlation between nocturnal intermittent hypoxemia and mild cognitive impairment in the older adult and the role of BDNF Val66Met polymorphism: a hospital-based cross-sectional study
影响因子:2.655刊物:Sleep and Breathing发表日期:2022/12/26 - The Effect of sequential bilateral low-frequency rTMS over dorsolateral prefrontal cortex on serum level of BDNF and GABA in patients with primary insomnia
影响因子:2.072刊物:Brain and Behavior发表日期:2019/1/4 - Effects of Training with a Brain–Computer Interface-Controlled Robot on Rehabilitation Outcome in Patients with Subacute Stroke: A Randomized Controlled Trial
影响因子:4.446刊物:Neurology and Therapy发表日期:2022/2/16 - Analgesic Effects of Navigated Repetitive Transcranial Magnetic Stimulation in Patients With Acute Central Poststroke Pain
影响因子:5.725刊物:Pain and Therapy发表日期:2021/4/17 - Using Dual-Target rTMS, Single-Target rTMS, or Sham rTMS on Post-Stroke Cognitive Impairment
影响因子:2.5刊物:Journal of Integrative Neuroscience发表日期:2024-08-23 - PM2.5 exposure upregulates pro-inflammatory protein expression in human microglial cells via oxidant stress and TLR4/NF-κB pathway
影响因子:6.2刊物:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY发表日期:2024-04-23 - Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis
影响因子:6刊物:AMERICAN JOURNAL OF PATHOLOGY发表日期:2023-10-31 - Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression
影响因子:3.5刊物:Journal of Dental Sciences发表日期:2023-06-25