T cell exhaustion is the main cause of sepsis -induced immunosuppression, and is associated with the poor prognosis. Nicotinamide adenine dinucleotide (NAD + ) is well known for its anti-aging effect, but its role in sepsis -induced T cell exhaustion remains to be elucidated. In the present study, using a classic septic animal model, we found that the levels of NAD + and its downstream molecule—sirtuins 1 ( SIRT1 ) in T cells in sepsis were decreased. Supplementation with Nicotinamide ribose (NR), the precursor of NAD + , right after cecal ligation and puncture significantly increased the levels of NAD + and SIRT1 . Supplementation with NR alleviated the depletion of mononuclear cells and T lymphocytes in spleen in sepsis , and increased the levels of CD3 + CD4 + and CD3 + CD8 + T cells . Interestingly, both Th1 and Th2 cells were expanded after NR treatment, but the balance of Th1/Th2 was partly restored. NR also inhibited the regulatory T cells expansion and PD-1 expression in CD4 + T cells in sepsis . Additionally, the bacteria load, organ damage (lung, heart, liver and kidney) and the mortality of septic mice were reduced after NR supplementation. In summary, these results demonstrate the beneficial effect of NR on sepsis and T cell exhaustion, which is associated with NAD + / SIRT1 pathway.
文章引用产品列表
-
- EK282 1454 Citations
- ELISA试剂盒
Mouse TNF-a ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥10,800.00
-
- EK282HS 1241 Citations
- 高敏试剂盒
Mouse TNF-α High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥2,000.00 – ¥3,400.00
-
- EK210 486 Citations
- ELISA试剂盒
Mouse IL-10 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥10,800.00
-
- EK206 1296 Citations
- ELISA试剂盒
Mouse IL-6 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥10,800.00
-
- EK206HS 1080 Citations
- 高敏试剂盒
Mouse IL-6 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥2,000.00 – ¥3,400.00