EK206

Mouse IL-6 ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

  • 分子靶点:IL6, IL-6, IFNB2, HGF
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:10 μL
  • 灵敏度:0.56 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:89% - 112%

在售SKU:70-EK206/3-48, 70-EK206/3-96


描述

商品名

Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.3% - 3.0%;板间变异系数:2.1% - 5.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

10 μL

灵敏度

0.56 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

89% - 112%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-6冻干标准品
  • IL-6检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名:IL6, interleukin 6
  • 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名:IL-6; BSF2; HGF; HSF
  • 曾用名:IFNB2
  • 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

小鼠 Mouse Il6 分子靶点信息

小鼠 Mouse Il6 靶点分子功能(预测)

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

操作步骤


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