EK206

Mouse IL-6 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

  • 分子靶点：IL6, IL-6, IFNB2, HGF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：10 μL
  • 灵敏度：0.56 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：89% - 112%

在售SKU:70-EK206/3-48, 70-EK206/3-96

描述

商品名

Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.3% - 3.0%；板间变异系数：2.1% - 5.3%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

10 μL
灵敏度

0.56 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

89% - 112%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-6冻干标准品
  • IL-6检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

小鼠 Mouse Il6 分子靶点信息

小鼠 Mouse Il6 靶点分子功能（预测）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA系列 | 血管内皮生长因子（VEGF） 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

直播预告 | ELISA实验技能要点与常见问题解析 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献


  1. 小鼠成软骨细胞 ATDC5 组织培养上清 细胞培养上清

    An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment 

  2. 细胞培养上清

    Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer 

  3. 血清

    Tumor-Selective Activation of Toll-Like Receptor 7/8 Agonist Nano-Immunomodulator Generates Safe Anti-Tumor Immune Responses upon Systemic Administration 

  4. 海马体 组织匀浆

    Multifunctional Nanostructure Rap-Rl Rescues Alzheimer's Cognitive Deficits through Remodeling the Neurovascular Unit 

  5. 细胞培养上清 血清

    The Immunoenhancement Effects of Polyethylenimine-Modified Chinese Yam Polysaccharide-Encapsulated Plga Nanoparticles as an Adjuvant 

  6. RAW 264.7 细胞培养上清

    Immunomodulatory Effects of Fucosylated Chondroitin Sulfate from Stichopus Chloronotus on Raw 264.7 Cells 

  7. 组织匀浆 脾脏 血清

    Administration Routes of Polyethylenimine-Coated Plga Nanoparticles Encapsulating Angelica Sinensis Polysaccharide Vaccine Delivery System Affect Immune Responses 

  8. 组织培养上清 细胞培养上清 脐带静脉内皮细胞 血浆 血管

    Il (Interleukin)-6 Contributes to Deep Vein Thrombosis and Is Negatively Regulated by Mir-338-5p 

  9. RAW 264.7 单核巨噬细胞 细胞培养上清

    Haimufang Decoction, a Chinese Medicine Formula for Lung Cancer, Arrests Cell Cycle, Stimulates Apoptosis in Nci-H1975 Cells, and Induces M1 Polarization in Raw 264.7 Macrophage Cells 

  10. 细胞培养上清

    Polylysine-Bilirubin Conjugates Maintain Functional Islets and Promote M2 Macrophage Polarization 

  11. 组织匀浆 结肠

    Genipin-Crosslinked Human Serum Albumin Coating Using a Tannic Acid Layer for Enhanced Oral Administration of Curcumin in the Treatment of Ulcerative Colitis 

  12. 支气管肺泡灌洗液 BALF 细胞培养上清 血清

    Paclitaxel Alleviated Sepsis-Induced Acute Lung Injury by Activating Muc1 and Suppressing Tlr-4/Nf-Κb Pathway 

  13. 组织匀浆 脑脊液 脾脏 血清

    Biomimetic Lipopolysaccharide-Free Bacterial Outer Membrane-Functionalized Nanoparticles for Brain-Targeted Drug Delivery 

  14. 血清

    Sodium Butyrate Ameliorates Schistosoma Japonicum-Induced Liver Fibrosis by Inhibiting Hmgb1 Expression 

  15. RAW 264.7 细胞培养上清

    A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis Indigotica 

  16. 组织匀浆

    Entrapment of Macrophage-Target Nanoparticles by Yeast Microparticles for Rhein Delivery in Ulcerative Colitis Treatment 

  17. 细胞培养上清 血清

    Sars-Cov-2 N Protein Promotes Nlrp3 Inflammasome Activation to Induce Hyperinflammation 

  18. 组织匀浆 细胞培养上清 结肠

    Oral Core-Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy 

  19. 血清

    A Small and Highly Sensitive Red/Far-Red Optogenetic Switch for Applications in Mammals 

  20. 血清

    Living Bacterial Hydrogels for Accelerated Infected Wound Healing 

  21. 细胞培养上清

    Polarization of Tumor-Associated Macrophages by Nanoparticle-Loaded Escherichia Coli Combined with Immunogenic Cell Death for Cancer Immunotherapy 

  22. 支气管肺泡灌洗液 BALF 组织匀浆

    Capsaicin Protects against Lipopolysaccharide-Induced Acute Lung Injury through the Hmgb1/Nf-Κb and Pi3k/Akt/Mtor Pathways 

  23. 支气管肺泡灌洗液 BALF 血清

    Syndecan-1 Shedding by Matrix Metalloproteinase-9 Signaling Regulates Alveolar Epithelial Tight Junction in Lipopolysaccharide-Induced Early Acute Lung Injury 

  24. 组织匀浆 细胞培养上清

    Protective Effects of Chitosan-Bilirubin Nanoparticles against Ethanol-Induced Gastric Ulcers 

  25. 组织匀浆 细胞培养上清 肿瘤 血清

    Bacterium-Mimicking Vector with Enhanced Adjuvanticity for Cancer Immunotherapy and Minimized Toxicity 

  26. 细胞培养上清

    Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 

  27. 血清

    Comparison of CD146 +/- mesenchymal stem cells in improving premature ovarian failure 

  28. RAW 264.7 细胞培养上清

    The activation effects of fucoidan from sea cucumber Stichopus chloronotus on RAW264.7 cells via TLR2/4-NF-κB pathway and its structure-activity relationship 

  29. 血清

    Spatiotemporally Controllable Distribution of Combination Therapeutics in Solid Tumors by Dually Modified Bacteria 

  30. 组织匀浆 肺 血清

    Non-peptidyl non-covalent cathepsin C inhibitoEEr bearing a unique thiophene-substituted pyridine: Design, structure-activity relationship and anti-inflammatory activity in vivo 

  31. 血清

    Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice 

  32. 细胞培养上清

    Uncovering the Metabolic Mechanism of Salidroside Alleviating Microglial Hypoxia Inflammation Based on Microfluidic Chip-Mass Spectrometry 

  33. 全血 心组织 组织匀浆 细胞培养上清

    Red Blood Cell Membrane-Camouflaged PLGA Nanoparticles Loaded With Basic Fibroblast Growth Factor for Attenuating Sepsis-Induced Cardiac Injury 

  34. 细胞培养上清

    Overcoming resistance to oncolytic virus M1 by targeting PI3K-γ in tumor-associated myeloid cells 

  35. RAW 264.7 细胞培养上清

    Two glycoproteins from medicinal insect Periplaneta americana (L.) promote diabetic wound healing via macrophage polarization modulation 

  36. 血清

    Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles 

  37. Oral colon-targeted mucoadhesive micelles with enzyme-responsive controlled release of curcumin for ulcerative colitis therapy 

  38. 细胞培养上清

    Neutrophil membrane-coated therapeutic liposomes for targeted treatment in acute lung injury 

  39. 细胞培养上清

    An Integration of Network Pharmacology and Experimental Verification to Investigate the Mechanism of Guizhi to Treat Nephrotic Syndrome 

  40. RAW 264.7

    8:2 Fluorotelomer Alcohol Inhibited Proliferation and Disturbed the Expression of Pro-Inflammatory Cytokines and Antigen-Presenting Genes in Murine Macrophages 

  41. 1, 8-Cineol Protect against Influenza-Virus-Induced Pneumonia in Mice 

  42. Silencing of Lncrna Steroid Receptor Rna Activator Attenuates Polycystic Ovary Syndrome in Mice 

  43. RAW 264.7

    I(4), a Synthetic Anti-Diabetes Agent, Attenuates Atherosclerosis through Its Lipid-Lowering, Anti-Inflammatory and Anti-Apoptosis Properties 

  44. Characterization and Immunomodulatory Activity of Polysaccharides from the Stems and Leaves of Abelmoschus Manihot and a Sulfated Derivative 

  45. RAW 264.7

    Semi-Mechanism-Based Pharmacodynamic Model for the Anti-Inflammatory Effect of Baicalein in Lps-Stimulated Raw264.7 Macrophages 

  46. Targeting Tumor Microenvironment: Effects of Chinese Herbal Formulae on Macrophage-Mediated Lung Cancer in Mice 

  47. RAW 264.7

    Asiatic Acid Attenuates Lipopolysaccharide-Induced Injury by Suppressing Activation of the Notch Signaling Pathway 

  48. Strigolactones: A Plant Phytohormone as Novel Anti-Inflammatory Agents 

  49. 细胞培养上清 血清

    Metformin protects against intestinal barrier dysfunction via AMPKα1-dependent inhibition of JNK signalling activation 

  50. 血清

    Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation 

评价

目前还没有评价

成为第一个“Mouse IL-6 ELISA Kit检测试剂盒（酶联免疫吸附法）” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注

相关产品