Rat IL-2 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK302HS-48, 70-EK302HS-96
- 分子靶点:IL2
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.71pg/mL
- 检测范围:12.5-800pg/mL
- 回收率:95%-119%
ELISA试剂盒详细信息
商品名 | 大鼠白介素2高敏酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | IL2 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.71pg/mL |
线性范围 | 12.5-800pg/mL |
精密度 | 板内变异系数:4.0%-4.4%;板间变异系数:3.9%-4.8% |
回收率 | 95%-119% |
平均回收率 | 103% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-2与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL2 分子靶点信息概述
- 分子名:IL2, interleukin 2
- 基因家族:Interleukins
- 别名:IL-2; TCGF
- 全称:T cell growth factor
IL2 分子靶点综述
白细胞介素2(IL-2)是15kDa的糖蛋白,在人类中,由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子,调控对具有免疫功能的白血球(白细胞,通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答,区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能,其受体由3条链组成,分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角,包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。
大鼠 Rat Il2 分子靶点信息
- 分子名:Il2, interleukin 2
- 别称:
- IL-2
- interleukin-2
- T-cell growth factor
- TCGF
- 基因序列:NCBI_Gene: 116562
- 蛋白序列:UniProtKB: P17108
大鼠 Rat Il2 靶点分子功能(预测)
Enables carbohydrate binding activity; glycosphingolipid binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of T cell activation; protein kinase C-activating G protein-coupled receptor signaling pathway; and regulation of protein phosphorylation. Located in extracellular space. Used to study cystitis. Biomarker of allergic rhinitis; colorectal carcinoma; and tongue squamous cell carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
引用文献统计
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