Mouse Granzyme B ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK2173-48, 70-EK2173-96, EK2173
- 分子靶点:GZMB
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:0.91pg/mL
- 检测范围:0.31-20ng/mL
- 回收率:91%-105%
ELISA试剂盒详细信息
商品名 | 小鼠颗粒酶B酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | GZMB |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 0.91pg/mL |
线性范围 | 0.31-20ng/mL |
精密度 | 板内变异系数:2.9%-3.9%;板间变异系数:1.8%-2.8% |
回收率 | 91%-105% |
平均回收率 | 99% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠GranzymeB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的GranzymeB与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GranzymeB的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
GZMB 分子靶点信息概述
- 分子名:GZMB, granzyme B
- 基因家族:Granule associated serine proteases of immune defence
- 别名:CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
- 曾用名:CTLA1; CSPB
- 全称:fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
GZMB 分子靶点综述
颗粒酶B(GranzymeB)是一种丝氨酸蛋白酶,最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)颗粒中。由这些细胞分泌的GranzymeB和穿孔蛋白一起作用介导靶细胞的凋亡。最近,人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生GranzymeB。GranzymeB通过刺激细胞因子的释放参与诱导炎症,同样也参与细胞外基质重塑。升高的GranzymeB水平与许多自身免疫性疾病、一些皮肤病和1型糖尿病有关。
小鼠 Mouse Gzme 分子靶点信息
- 分子名:Gzme, granzyme E
- 别称:
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
- 基因序列:NCBI_Gene: 14942
- 蛋白序列:
小鼠 Mouse Gzme 靶点分子功能(预测)
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).
引用文献统计
该产品被引用的文献总数为:27
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