EasyGo!™ Human/Mouse/Rat TGF-β1 ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥2,190.00 – ¥3,650.00
在售SKU:70-EK981EGA-48, 70-EK981EGA-96, EK981EGA
- 分子靶点:TGFB1
- 种属:人、小鼠、大鼠
- 样本类型:人、大鼠、小鼠血清
- 检测样本体积:血清、血浆样本40μL
- 灵敏度:10.76pg/mL
- 检测范围:15.625-1000pg/mL
- 回收率:75%-109%
ELISA试剂盒详细信息
商品名 | EasyGo!™ 人/小鼠/大鼠TGF-β1 酶联免疫检测试剂盒 |
---|---|
种属 | 人、小鼠、大鼠 |
靶点 | TGFB1 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 人、大鼠、小鼠血清 |
检测样本体积 | 血清、血浆样本40μL |
灵敏度 | 10.76pg/mL |
线性范围 | 15.625-1000pg/mL |
精密度 | 板内变异系数:5.8%-9.1%;板间变异系数:4.3%-6.9% |
回收率 | 75%-109% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入生物素标记的检测抗体微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长 (参考波长570 - 630nm) 测定吸光度值。 |
分子信息
TGFB1 分子靶点信息概述
- 分子名:TGFB1, transforming growth factor beta 1
- 基因家族:Transforming growth factor beta family
- 别名:CED; TGFbeta
- 曾用名:TGFB; DPD1
- 全称:Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1
TGFB1 分子靶点综述
转化生长因子β1 (TGF-β1)是转化生长因子β超家族的多肽成员,具有多种细胞学功能,包括调控细胞生长、增殖、分化和凋亡。TGF-β与TGFA协同诱导转化。它也能作为负向自分泌生长因子。TGF-β活化和信号传导的失调可能会引起凋亡。很多细胞可合成TGF-β,且几乎都有特异性的受体。TGF-β1、TGF-β2和TGF-β3都通过相同的受体信号通路发挥功能。TGF-β1对调控免疫系统具有重要作用,对不同类型的细胞或不同发育阶段的细胞具有不同的活性。大部分免疫细胞 (或白细胞) 分泌TGF-β1。TGF-β1与癌症、自身免疫疾病、肝脏疾病、肾脏疾病、糖尿病、心血管疾病、哮喘、慢性阻塞性肺疾病 (COPD)和囊肿性纤维化 (CF) 等相关。
人 Human TGFB1 分子靶点信息
- 分子名:TGFB1, transforming growth factor beta 1
- 别称:
- CED
- DPD1
- IBDIMDE
- LAP
- latency-associated peptide
- prepro-transforming growth factor beta-1
- TGF-beta 1 protein
- TGF-beta-1
- TGF-beta1
- TGFB
- TGFbeta
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor beta1
- 基因序列:NCBI_Gene: 7040
- 蛋白序列:UniProtKB: P01137
人 Human TGFB1靶点分子功能(预测)
Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet's disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).