EK280

Mouse IFN-gamma ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

  • 分子靶点：IFNG, IFN-γ
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.83 pg/mL
  • 检测范围：23.44 pg/mL - 1500 pg/mL
  • 回收率：90% - 108%

在售SKU:70-EK280/3-48, 70-EK280/3-96

商品名

Mouse IFN-γ ELISA Kit (小鼠γ干扰素 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.6% - 7.0%；板间变异系数：3.2% - 4.7%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.83 pg/mL
检测范围

23.44 pg/mL - 1500 pg/mL
回收率

90% - 108%
平均回收率

1.01
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IFN-γ单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IFN-γ冻干标准品
  • IFN-γ检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

分子信息

概述小鼠 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名：IFNG, interferon gamma
  • 基因家族：Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。

小鼠 Mouse Ifng 分子靶点信息

小鼠 Mouse Ifng 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of generation of precursor metabolites and energy. Acts upstream of or within several processes, including CD8-positive, alpha-beta T cell differentiation involved in immune response; defense response to other organism; and positive regulation of macromolecule metabolic process. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study several diseases, including aplastic anemia; hepatitis; malaria; medulloblastoma; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including allergic rhinitis; ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

引用文献统计

该产品被引用的文献总数为：477

ELISA试剂盒操作常见问题

小心！你的 ELISA 试剂盒可能是假的

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞

开学学习计划，如何收集ELISA检测样本——细胞 

开学学习计划，如何选择ELISA试剂盒

开学学习计划，如何选择ELISA试剂盒 

开学学习计划，ELISA从新手到入门！

开学学习计划，ELISA从新手到入门！ 

一文掌握ELISA实验显色判断、数据分析及标曲拟合

一文掌握ELISA实验显色判断、数据分析及标曲拟合 

血清OR血浆，哪个是ELISA的菜

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒

ELISA通关必备丨如何选择试剂盒 

查看更多ELISA试剂盒操作步骤技术文章

操作步骤

ELISA操作常见问题

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞 

开学学习计划，如何选择ELISA试剂盒 

开学学习计划，ELISA从新手到入门！ 

一文掌握ELISA实验显色判断、数据分析及标曲拟合 

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. A pH-responsive polymersome depleting regulatory T cells and blocking A2A receptor for cancer immunotherapy 

  2. Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. 

  3. A light-driven dual-nanotransformer with deep tumor penetration for efficient chemo-immunotherapy 

  4. In vivo effect of magnetic microspheres loaded with E2-a in the treatment of alveolar echinococcosis 

  5. Melatonin regulates the immune response and improves Sjögren's syndrome-like symptoms in NOD/Ltj Mice. 

  6. Locally organised and activated Fth1hi neutrophils aggravate inflammation of acute lung injury in an IL-10-dependent manner 

  7. Effects of divalent cations on the physical, conformational and immunological properties of bovine allergen β-lactoglobulin aggregates 

  8. Phloretin inhibited the pathogenicity and virulence factors against Candida albicans 

  9. Alhagi honey polysaccharides encapsulated into PLGA nanoparticle-based pickering emulsion as a novel adjuvant to induce strong and long-lasting immune responses 

  10. Alpha radionuclide-chelated radioimmunotherapy promoters enable local radiotherapy/chemodynamic therapy to discourage cancer progression 

  11. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy 

  12. Bushen Jianpi Quyu Formula Alleviates Myelosuppression of an Immune-Mediated Aplastic Anemia Mouse Model via Inhibiting Expression of the PI3K/AKT/NF-κB Signaling Pathway 

  13. Tumor Microenvironment-Triggered Charge Reversal Polymetformin-Based Nanosystem Co-Delivered Doxorubicin and IL-12 Cytokine Gene for Chemo–Gene Combination Therapy on Metastatic Breast Cancer 

  14. Sophocarpine Protects Mice from ConA-Induced Hepatitis via Inhibition of the IFN-Gamma/STAT1 Pathway 

  15. A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases 

  16. The structures of two glucomannans from Bletilla formosana and their protective effect on inflammation via inhibiting NF-κB pathway 

  17. Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis 

  18. Crizotinib prodrug micelles co-delivered doxorubicin for synergistic immunogenic cell death induction on breast cancer chemo-immunotherapy 

  19. A general strategy towards an injectable microwave-sensitive immune hydrogel for combined percutaneous microwave ablation and immunotherapy 

  20. Microneedle-Assisted, DC-Targeted Codelivery of pTRP-2 and Adjuvant of Paclitaxel for Transcutaneous Immunotherapy 

  21. Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model 

  22. Self-Assembled Immunostimulatory Tetrahedral Framework Nucleic Acid Vehicles for Tumor Chemo-immunotherapy 

  23. Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice 

  24. GPX4-Regulated Ferroptosis Mediates S100-Induced Experimental Autoimmune Hepatitis Associated with the Nrf2/HO-1 Signaling Pathway 

  25. A small and highly sensitive red/far-red optogenetic switch for applications in mammals 

  26. Sendai virus acts as a nano-booster to excite dendritic cells for enhancing the efficacy of CD47-directed immune checkpoint inhibitors against breast carcinoma 

  27. Light-Triggered Efficient Sequential Drug Delivery of Biomimetic Nanosystem for Multimodal Chemo-, Antiangiogenic, and Anti-MDSC Therapy in Melanoma 

  28. Sodium butyrate ameliorates Schistosoma japonicum-induced liver fibrosis by inhibiting HMGB1 expression 

  29. Dimethyl fumarate inhibits antibody-induced platelet destruction in immune thrombocytopenia mouse 

  30. Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer 

  31. Synthetic Lignin-Derived Therapeutic Nano Reagent as Intestinal pH-Sensitive Drug Carriers Capable of Bypassing the Gastric Acid Environment for Colitis Treatment 

  32. Define Mesenchymal Stem Cell from Its Fate: Biodisposition of Human Mesenchymal Stem Cells in Normal and Concanavalin A-Induced Liver Injury Mice. 

  33. A Nanoplatform to Amplify Apoptosis-to-Pyroptosis Immunotherapy via Immunomodulation of Myeloid-Derived Suppressor Cells 

  34. Exosomes released by granulocytic myeloid-derived suppressor cells attenuate DSS-induced colitis in mice 

  35. On-demand integrated nano-engager converting cold tumors to hot via increased DNA damage and dual immune checkpoint inhibition 

  36. METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells 

  37. Improved immune response to an attenuated pseudorabies virus vaccine by ginseng stem-leaf saponins (GSLS) in combination with thimerosal (TS) 

  38. Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape 

  39. Adjuvant effect of two polysaccharides from the petals of Crocus sativus and its mechanisms 

  40. Augmenter of liver regeneration (ALR) restrains concanavalin A-induced hepatitis in mice 

  41. Low-temperature photothermal irradiation triggers alkyl radicals burst for potentiating cancer immunotherapy 

  42. Rocaglamide Prolonged Allograft Survival by Inhibiting Differentiation of Th1/Th17 Cells in Cardiac Transplantation 

  43. A Checkpoint-Regulatable Immune Niche Created by Injectable Hydrogel for Tumor Therapy 

  44. Loss of fragile site-associated tumor suppressor promotes antitumor immunity via macrophage polarization 

  45. Laser/GSH-Activatable Oxaliplatin/Phthalocyanine-Based Coordination Polymer Nanoparticles Combining Chemophotodynamic Therapy to Improve Cancer Immunotherapy 

  46. Chinese Patent Medicine Liuweiwuling Tablet had Potent Inhibitory Effects on Both Wild-Type and Entecavir-Resistant Hepatitis B Virus (HBV) in vitro and Effectively Suppressed HBV Replication in Mouse Model 

  47. The therapeutic effect of an autologous and allogenic mixed glioma cell lysate vaccine in a rat model 

  48. Oral Probiotics Ameliorate the Behavioral Deficits Induced by Chronic Mild Stress in Mice via the Gut Microbiota-Inflammation Axis 

  49. Multifunctional PVCL nanogels enable magnetic resonance imaging and immunostimulated radiotherapy of orthotopic glioblastoma 

  50. Synergistic effects of low‑dose chemotherapy and T cells in renal cell carcinoma 

  51. Cytokine-induced killer cells-assisted tumor-targeting delivery of Her-2 monoclonal antibody-conjugated gold nanostars with NIR photosensitizer for enhanced therapy of cancer 

  52. Down-regulation of taurine-up-regulated gene 1 attenuates inflammation by sponging miR-9-5p via targeting NF-κB1/p50 in multiple sclerosis 

  53. Erythrocyte Membrane-Enveloped Polymeric Nanoparticles as Nanovaccine for Induction of Antitumor Immunity against Melanoma 

  54. Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD 

  55. Tri-component programmable nanoregulator with Three-pronged penetration boosts immunotherapy of Triple-Negative breast cancer 

  56. Administration Routes of Polyethylenimine-Coated PLGA Nanoparticles Encapsulating Angelica Sinensis Polysaccharide Vaccine Delivery System Affect Immune Responses 

  57. The Therapeutic Effects of Baicalin on Vitiligo Mice 

  58. Plant-derived RNAi therapeutics: A strategic inhibitor of HBsAg 

  59. A neonatal murine model of coxsackievirus A4 infection for evaluation of vaccines and antiviral drugs 

  60. Immune-enhancing effects of polysaccharides MLN-1 from by-product of Mai-luo-ning in vivo and in vitro 

  61. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice 

  62. The therapeutic effects of EGCG on vitiligo 

  63. RETRACTED ARTICLE: Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice 

  64. Antitumor efficacy of chimeric antigen receptor T cells against EGFRvIII-expressing glioblastoma in C57BL/6 mice 

  65. Protection against Mycobacterium tuberculosis Infection Offered by a New Multistage Subunit Vaccine Correlates with Increased Number of IFN-γ+IL-2+ CD4+ and IFN-γ+ CD8+ T Cells 

  66. Dioxin-like (DL-) polychlorinated biphenyls induced immunotoxicity through apoptosis in mice splenocytes via the AhR mediated mitochondria dependent signaling pathways 

  67. Codelivered Chemotherapeutic Doxorubicin via a Dual-Functional Immunostimulatory Polymeric Prodrug for Breast Cancer Immunochemotherapy 

  68. Structural characterization of a novel Schisandra polysaccharides and nutritional intervention in immunotoxicity to PCBs 

  69. Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade 

  70. Macrophage immunomodulatory activity of the cationic polymer modified PLGA nanoparticles encapsulating Alhagi honey polysaccharide 

  71. 8:2 Fluorotelomer alcohol causes immunotoxicity and liver injury in adult male C57BL/6 mice 

  72. Effect of the Chinese Medicine YangZheng XiaoJi on Reducing Fatigue in Mice with Orthotopic Transplantation of Colon Cancer 

  73. Camouflaging bacteria by wrapping with cell membranes 

  74. GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 

  75. A Novel Tail-Associated O91-Specific Polysaccharide Depolymerase from a Podophage Reveals Lytic Efficacy of Shiga Toxin-Producing Escherichia coli 

  76. Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases 

  77. S-allylmercaptocysteine ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting inflammation and oxidative stress via nuclear factor kappa B and Keap1/Nrf2 pathways 

  78. Enhanced antitumor activity of gemcitabine by polysaccharide-induced NK cell activation and immune cytotoxicity reduction in vitro/vivo 

  79. Dual polymeric prodrug co-assembled nanoparticles with precise ratiometric co-delivery of cisplatin and metformin for lung cancer chemoimmunotherapy 

  80. Blockage of the IDO1 pathway by charge-switchable nanoparticles amplifies immunogenic cell death for enhanced cancer immunotherapy 

  81. Targeting pulmonary tumor microenvironment with CXCR4-inhibiting nanocomplex to enhance anti–PD-L1 immunotherapy 

  82. Biomimetic radiosensitizers unlock radiogenetics for local interstitial radiotherapy to activate systematic immune responses and resist tumor metastasis 

  83. Tumor-Targeted Gene Silencing IDO Synergizes PTT-Induced Apoptosis and Enhances Anti-tumor Immunity 

  84. Therapeutic dendritic cell vaccines engineered with antigen-biomineralized Bi2S3 nanoparticles for personalized tumor radioimmunotherapy 

  85. Cryptotanshinone ameliorates the pathogenesis of systemic lupus erythematosus by blocking T cell proliferation 

  86. Simultaneous innate immunity activation and immunosuppression improvement by biodegradable nanoplatform for boosting antitumor chemo-immunotherapy 

  87. IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4+ T cells 

  88. A Vaccination with Boosted Cross Presentation by ER-Targeted Antigen Delivery for Anti-Tumor Immunotherapy 

  89. T-cell-specific Sel1L deletion exacerbates EAE by promoting Th1/Th17-cell differentiation 

  90. Mannose-Modified PLGA Nanoparticles for Sustained and Targeted Delivery in Hepatitis B Virus Immunoprophylaxis 

  91. Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation 

  92. Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization 

  93. Yifei Tongluo, a Chinese Herbal Formula, Suppresses Tumor Growth and Metastasis and Exerts Immunomodulatory Effect in Lewis Lung Carcinoma Mice 

  94. Prolonging Survival of Corneal Transplantation by Selective Sphingosine-1-Phosphate Receptor 1 Agonist 

  95. Systemic application of sphingosine 1-phosphate receptor 1 immunomodulator inhibits corneal allograft rejection in mice 

  96. A new antisarcoma strategy: multisubtype heat shock protein/peptide immunotherapy combined with PD-L1 immunological checkpoint inhibitors 

  97. LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization 

  98. Bacteroides thetaiotaomicron relieves colon inflammation by activating aryl hydrocarbon receptor and modulating CD4+T cell homeostasis 

  99. Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice 

  100. The interaction between STAT3 and nAChRα1 interferes with nicotine-induced atherosclerosis via Akt/mTOR signaling cascade 

  101. TREM-2 Promotes Host Resistance Against Pseudomonas aeruginosa Infection by Suppressing Corneal Inflammation via a PI3K/Akt Signaling Pathway 

  102. Oxygen-carrying nanoparticle-based chemo-sonodynamic therapy for tumor suppression and autoimmunity activation 

  103. A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice 

  104. Impaired CCR9/CCL25 signalling induced by inefficient dendritic cells contributes to intestinal immune imbalance in nonalcoholic steatohepatitis 

  105. Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant 

  106. Ginseng Stem-Leaf Saponins in Combination with Selenium Promote the Immune Response in Neonatal Mice with Maternal Antibody 

  107. Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment 

  108. Effects of Acetylshikonin on the Infection and Replication of Coxsackievirus A16 in Vitro and in Vivo 

  109. Methylprednisolone Decreases Mitochondria-Mediated Apoptosis and Autophagy Dysfunction in Hepatocytes of Experimental Autoimmune Hepatitis Model via the Akt/mTOR Signaling 

  110. Long non-coding RNA growth arrest specific-5: a potential biomarker for early diagnosis of severe asthma 

  111. HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 

  112. Engineering ApoE3-incorporated biomimetic nanoparticle for efficient vaccine delivery to dendritic cells via macropinocytosis to enhance cancer immunotherapy 

  113. Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4+ T Cells 

  114. Effects and mechanism of Chinese medicine Jiawei Yupingfeng in a mouse model of allergic rhinitis 

  115. Morphine induces the differentiation of T helper cells to Th2 effector cells via the PKC-θ–GATA3 pathway 

  116. Olaparib Suppresses MDSC Recruitment via SDF1α/CXCR4 Axis to Improve the Anti-tumor Efficacy of CAR-T Cells on Breast Cancer in Mice 

  117. Mucosal immunity of mannose-modified chitosan microspheres loaded with the nontyepable Haemophilus influenzae outer membrane protein P6 in BALB/c mice 

  118. Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion 

  119. Combined Adjuvant of Poly I:C Improves Antitumor Effects of CAR-T Cells 

  120. Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation 

相关产品