Human IL-10 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK110HS-48, 70-EK110HS-96, 人白细胞介素10, 白细胞介素10, 白细胞介素, 人, 白介素, 10, 高敏
- 分子靶点:IL10
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本50μL
- 灵敏度:0.05pg/mL
- 检测范围:0.39-25pg/mL
- 回收率:88%-100%
ELISA试剂盒详细信息
商品名 | 人白介素10高敏酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | IL10 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本50μL |
灵敏度 | 0.05pg/mL |
线性范围 | 0.39-25pg/mL |
精密度 | 板内变异系数:3.5%-4.1%;板间变异系数:4.0%-8.8% |
回收率 | 88%-100% |
平均回收率 | 92% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-10与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL10 分子靶点信息概述
- 分子名:IL10, interleukin 10
- 基因家族:Interleukins
- 别名:CSIF; TGIF; IL10A; IL-10
- 全称:cytokine synthesis inhibitory factor; T-cell growth inhibitory factor
IL10 分子靶点综述
白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中,它由位于1号染色体的IL-10基因编码,由5个外显子组成。IL-10主要由单核细胞分泌,淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性,并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床,抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。
人 Human IL10 分子靶点信息
- 分子名:IL10, interleukin 10
- 别称:
- CSIF
- cytokine synthesis inhibitory factor
- GVHDS
- IL-10
- IL10A
- interleukin-10
- MGC126450
- MGC126451
- T-cell growth inhibitory factor
- TGIF
- 基因序列:NCBI_Gene: 3586
- 蛋白序列:UniProtKB: P22301
人 Human IL10靶点分子功能(预测)
Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).
引用文献统计
该产品被引用的文献总数为:115
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