EK110

Human IL-10 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

在售SKU:70-EK110/2-48, 70-EK110/2-96, EK110, 人白细胞介素10, 白细胞介素10, 白细胞介素, 10, 白介素, EK110, EK110, EK110, EK110, EK110

  • 分子靶点：IL10
  • 种属：人
  • 样本类型：血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：50μL；细胞培养上清：100μL
  • 灵敏度：0.43pg/mL
  • 检测范围：3.13-200pg/mL
  • 回收率：81%-103%

ELISA试剂盒详细信息

商品名人白介素10酶联免疫检测试剂盒
种属人
靶点IL10
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清血浆：50μL；细胞培养上清：100μL
灵敏度0.43pg/mL
线性范围3.13-200pg/mL
精密度板内变异系数：3.2%-5.7%;板间变异系数：2.9%-8.2%
回收率81%-103%
平均回收率91%
板式96孔板，可拆
保存条件2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述人 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

人 Human IL10 分子靶点信息

  • 分子名：IL10, interleukin 10
  • 别称：
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • 基因序列：NCBI_Gene: 3586
  • 蛋白序列：UniProtKB: P22301

人 Human IL10靶点分子功能（预测）

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

引用文献统计

该产品被引用的文献总数为：138

ELISA试剂盒操作常见问题

小心！你的 ELISA 试剂盒可能是假的

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞

开学学习计划，如何收集ELISA检测样本——细胞 

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开学学习计划，如何选择ELISA试剂盒 

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开学学习计划，ELISA从新手到入门！ 

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血清OR血浆，哪个是ELISA的菜 

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ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒

ELISA通关必备丨如何选择试剂盒 

查看更多ELISA试剂盒操作步骤技术文章

操作步骤

ELISA操作常见问题

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞 

开学学习计划，如何选择ELISA试剂盒 

开学学习计划，ELISA从新手到入门！ 

一文掌握ELISA实验显色判断、数据分析及标曲拟合 

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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引用文献

文章目录[隐藏]

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  40. Effects of high serum uric acid levels on oxidative stress levels and semen parameters in male infertile patients 

  41. Increased Tim-3+ monocytes/macrophages are associated with disease severity in patients with IgA nephropathy 

  42. Lipopolysaccharide from biofilm-forming Pseudomonas aeruginosa PAO1 induces macrophage hyperinflammatory responses 

  43. Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 

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