EK2127

Mouse BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK2127-48, 70-EK2127-96, EK2127

  • 分子靶点:BDNF
  • 种属:小鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本10μL
  • 灵敏度:1.05pg/mL
  • 检测范围:15.63-1000pg/mL
  • 回收率:75%-126%

96T

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ELISA试剂盒详细信息

商品名小鼠脑源性神经营养因子酶联免疫检测试剂盒
种属小鼠
靶点BDNF
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本10μL
灵敏度1.05pg/mL
线性范围15.63-1000pg/mL
精密度板内变异系数:2.7%-4.4%;板间变异系数:3.9%-4.9%
回收率75%-126%
平均回收率101%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述小鼠 BDNF, neurotrophin 靶点信息

BDNF 分子靶点信息概述

  • 分子名:BDNF, brain derived neurotrophic factor
  • 基因家族:Neurotrophins
  • 全称:neurotrophin

BDNF 分子靶点综述

脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。

小鼠 Mouse Bdnf 分子靶点信息

小鼠 Mouse Bdnf 靶点分子功能(预测)

Predicted to enable growth factor activity; nerve growth factor receptor binding activity; and neurotrophin TRKB receptor binding activity. Involved in negative regulation of apoptotic signaling pathway; positive regulation of neuron projection development; and retrograde trans-synaptic signaling by neuropeptide, modulating synaptic transmission. Acts upstream of or within several processes, including generation of neurons; modulation of chemical synaptic transmission; and sensory organ development. Located in several cellular components, including dendrite; perinuclear region of cytoplasm; and secretory granule. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).