Effect of selenized Athyrium multidentatum (Doll.) Ching polysaccharides on TNF/MAPKs/NF-κB signaling pathways in RAW 264.7 cells

Polysaccharides from Athyrium multidentatum (Doll.) Ching (AMC) were fractionated and selenylated by HNO₃-Na₂SeO₃ method. Four selenized polysaccharides were synthesized and fully characterized by ICP-MS, UV, FT-IR, ¹H NMR, high-performance gel permeation chromatography, high-performance liquid chromatography (HPLC), and SEM. Chemical analysis demonstrated that the selenized polysaccharides were composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) with different molar ratios and molecular weights. The immunoactivity results showed that the selenized polysaccharides elevated cell proliferation, phagocytic activity, and promoted the production of TNF-α, IL-6, IL-1β, IFN-γ, IL-10, NO, and reactive oxygen species in RAW 264.7 cells, indicating marked immune enhancement activities. RT-qPCR and Western blot assays revealed that the selenized AMC polysaccharides upregulated the expression levels of many key factors involved in TNF, MAPKs, and NF-κB signaling pathways. TNF/MAPKs/NF-κB signaling pathways might play crucial roles in the immune function of selenized AMC polysaccharides.

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