EK108

Human IL-8 ELISA Kit (人白介素8 ELISA试剂盒)

¥1,600.00¥2,650.00

  • 分子靶点:CXCL8, IL-8, GCP1, NAP1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.78 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:87% - 111%

在售SKU:70-EK108-48, 70-EK108-96, 人白细胞介素8, 白细胞介素8, 白细胞介素, 人, 白介素, 8


描述

商品名

Human IL-8 ELISA Kit (人白介素8 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.5% - 6.5%;板间变异系数:4.0%-4.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.78 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

87% - 111%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-8单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-8冻干标准品
  • IL-8检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-8抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-8与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-8的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-8 ELISA Kit (人白介素8 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CXCL8, IL-8, GCP1, NAP1 靶点信息

CXCL8 分子靶点信息概述

  • 分子名:CXCL8, C-X-C motif chemokine ligand 8
  • 基因家族:Chemokine ligands; Interleukins
  • 别名:SCYB8; LUCT; LECT; MDNCF; TSG-1; IL-8; NAP-1; 3-10C; MONAP; AMCF-I; LYNAP; NAF; b-ENAP; GCP-1; K60; GCP1; NAP1
  • 曾用名:IL8
  • 全称:neutrophil-activating peptide 1; granulocyte chemotactic protein 1; monocyte-derived neutrophil chemotactic factor; lung giant cell carcinoma-derived chemotactic protein; tumor necrosis factor-induced gene 1; monocyte-derived neutrophil-activating peptide; lymphocyte derived neutrophil activating peptide; beta endothelial cell-derived neutrophil activating peptide; alveolar macrophage chemotactic factor I; interleukin 8; chemokine (C-X-C motif) ligand 8

CXCL8 分子靶点综述

白细胞介素8(IL-8)是由巨噬细胞和其它类型细胞如上皮细胞、气道平滑肌细胞和内皮细胞产生的趋化因子,在人类中,由IL8基因编码。IL-8可有效促进血管生成,在细支气管炎的发病机制中起重要作用。IL-8可通过对中性粒细胞的召集和脱颗粒来实现对炎症反应的调节,可作为结肠癌细胞株的自分泌生长因子来作用于结肠直肠癌。高水平的IL-8可减少精神分裂症患者抗精神病药的阳性反应。另外,IL-8与肥胖和囊性纤维化的发病机制也相关。

人 Human CXCL8 分子靶点信息

  • 分子名:CXCL8, C-X-C motif chemokine ligand 8
  • 别称:
    • alveolar macrophage chemotactic factor I
    • beta endothelial cell-derived neutrophil activating peptide
    • beta-thromboglobulin-like protein
    • C-X-C motif chemokine 8
    • chemokine (C-X-C motif) ligand 8
    • emoctakin
    • GCP-1
    • GCP1
    • granulocyte chemotactic protein 1
    • IL8
    • interleukin 8
    • interleukin-8
    • LECT
    • LUCT
    • lung giant cell carcinoma-derived chemotactic protein
    • lymphocyte derived neutrophil activating peptide
    • lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MONAP
    • monocyte-derived neutrophil chemotactic factor
    • monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP-1
    • NAP1
    • neutrophil-activating peptide 1
    • SCYB8
    • small inducible cytokine subfamily B, member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • tumor necrosis factor-induced gene 1
  • 基因序列:NCBI_Gene: 3576
  • 蛋白序列:UniProtKB: P10145

人 Human CXCL8靶点分子功能(预测)

Enables chemokine activity and interleukin-8 receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; induction of positive chemotaxis; and neutrophil chemotaxis. Acts upstream of or within negative regulation of cell adhesion molecule production; positive regulation of cellular biosynthetic process; and regulation of gene expression. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in several diseases, including autoimmune uveitis; bacterial gastritis; gastrointestinal system cancer (multiple); trachoma; and urinary tract infection. Biomarker of several diseases, including chikungunya; cholestasis (multiple); cystic fibrosis; liver disease (multiple); and tuberculosis (multiple).

操作步骤


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引用文献


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  3. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  4. 细胞培养上清

    Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 

  5. Antimicrobial Activities of Peptide Cbf-K(16) against Drug-Resistant Helicobacter Pylori Infection in Vitro and in Vivo 

  6. C. Butyricum Lipoteichoic Acid Inhibits the Inflammatory Response and Apoptosis in Ht-29 Cells Induced by S. Aureus Lipoteichoic Acid 

  7. Oral Delivery of Bifidobacterium Longum Expressing Α-Melanocyte-Stimulating Hormone to Combat Ulcerative Colitis 

  8. Somg-833, a Novel Selective C-Met Inhibitor, Blocks C-Met-Dependent Neoplastic Effects and Exerts Antitumor Activity 

  9. Fenofibrate, a Pparα Agonist, Protect Proximal Tubular Cells from Albumin-Bound Fatty Acids Induced Apoptosis Via the Activation of Nf-Kb 

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