【DESCREPTION】Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFN-γ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes.
【PRINCIPLE OF THE ASSAY】This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IFN-γ present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IFN-γ is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.
【标准曲线范围】3.13- 200 pg/ml
【回收率范围】73 %-137 %
【板间变异系数】3.7% - 4.4 %
【板内变异系数】2.4 % - 8.5 %