70-MJ101

Mitochondria Staining Kit(JC-1)线粒体膜电位检测试剂盒 125 Tests

¥1,120.00

在售SKU:70-MJ101, 凋亡, 细胞凋亡, 凋亡试剂盒, 细胞凋亡试剂盒, 凋亡检测, 细胞凋亡检测, 凋亡检测试剂盒, 线粒体, 膜电位, ,线粒体膜电位, 膜电位检测, 膜电位检测试剂盒, 线粒体膜, 线粒体膜检测, Mitochondria Staining, Annexin V, Annexin, Apoptosis

描述

产品简介

线粒体电化学势梯度(ΔΨ)丧失是凋亡早期的事件。本试剂盒提供一种快速方便的方法,利用阳离子亲脂性染料JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’- tetraethylbenzimidazolocarbocyanine iodide)检测活细胞线粒体膜电位的变化,适合于凋亡细胞的检测。在正常细胞中,由于电化学势梯度,JC-1积聚在线粒体基质中,形成红色荧光的聚集体。破坏线粒体膜电位的事件会阻止JC-1染料积聚在线粒体中,因此该染料会扩散到整个细胞,使红色荧光转变为绿色荧光(JC-1单体)。CCCP (carbonyl cyanide 3-chlorophenylhydrazone)可用于研究线粒体膜电位。JC-1在线粒体中的积聚依赖于电位,其发射光从绿色(~529 nm)迁移到红色(~590 nm)。因此,线粒体去极化时,红色/绿色荧光强度之比会下降。JC-1在多种类型的细胞包括肌细胞和神经元,以及完整的组织和分离的线粒体中都可以作为线粒体膜电位的指示剂。

产品特点

  • 即用型,使用方便
  • 凋亡检测结果准确
  • 价格便宜,常备现货

试剂盒组分

引用文献

1
Zhang Y J, Xiao Y Y, Ma Y, et al. ROS-mediated miR-21-5p regulates the proliferation and apoptosis of Cr(VI)-exposed L02 hepatocytes via targeting PDCD4[J]. Ecotoxicology and Environmental Safety, 2020 (IF:4.527)

查看原文
2
Xu L , Wu T , Lu S , et al. Mitochondrial superoxide contributes to oxidative stress exacerbated by DNA damage response in RAD51-depleted ovarian cancer cells[J]. Redox Biology, 2020, 36 (IF: 9.986)

查看原文
3
Zhou X L, Wan L, Xu Q R, et al. Notch signaling activation contributes to cardioprotection provided by ischemic preconditioning and postconditioning[J]. J TRANSL MED, 2013, 11: 251 - 260 (IF:3.459)

查看原文
4
Du K, Liang J, Wang Y, et al. Dual inhibition of topoisomerases I and IIα by ruthenium(II) complexes containing asymmetric tridentate ligands[J]. Dalton Transactions, 2014, 43 (IF:4.097)

查看原文
5
Zhang S W, Li Y L, He X G, et al. Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo[J]. Int J Nanomedicine, 2014, 9: 1931–1946 (IF:3.463)

查看原文
6
Mei J, Yang L Y, Lai L, et al. The interactions between CdSe quantum dots and yeast Saccharomyces cerevisiae: Adhesion of quantum dots to the cell surface and the protection effect of ZnS shell[J]. CHEMOSPHERE, 2014, 112: 92–99 (IF:3.137)

查看原文
7
Jiang Z, Li X, Lin Z, et al. Ethyl pyruvate reduces hepatic mitochondrial swelling and dysfunction in a rat model of sepsis.[J]. International Journal of Clinical & Experimental Pathology, 2015, 8(7): 7774 (IF:1.891)

查看原文
8
Lai L, Jin J C, Xu Z Q, et al. Necrotic cell death induced by the protein-mediated intercellular uptake of CdTe quantum dots[J]. Chemosphere, 2015 (IF:3.34)

查看原文
9
Hou X, Yu Y, Feng J, et al. Biochemical changes of salivary gland adenoid cystic carcinoma cells induced by SGI-1776[J]. Experimental Cell Research, 2017, 352(2): 403-411 (IF:3.378)

查看原文
10
Zhang Y, Xiao F, Liu X, et al. Cr(VI) induces cytotoxicity in vitro through activation of ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction via the PI3K/Akt signaling pathway[J]. Toxicology in Vitro, 2017, 41: 232-244 (IF:3.338)

查看原文

评价

目前还没有评价

成为第一个“Mitochondria Staining Kit(JC-1)线粒体膜电位检测试剂盒 125 Tests” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注