Human IFN-gamma ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥10,800.00
在售SKU:70-EK180-48, 70-EK180-96, EK180
- 分子靶点:IFNG
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:50μL;细胞培养上清:100μL
- 灵敏度:0.30pg/mL
- 检测范围:15.63-1000ng/mL
- 回收率:83%-113%
ELISA试剂盒详细信息
商品名 | 人γ-干扰素酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | IFNG |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清血浆:50μL;细胞培养上清:100μL |
灵敏度 | 0.30pg/mL |
线性范围 | 15.63-1000ng/mL |
精密度 | 板内变异系数:3.6%-4.5%;板间变异系数:4.5%-7.6% |
回收率 | 83%-113% |
平均回收率 | 95% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IFNG 分子靶点信息概述
- 分子名:IFNG, interferon gamma
- 基因家族:Interferons
IFNG 分子靶点综述
IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。
人 Human IFNG 分子靶点信息
- 分子名:IFNG, interferon gamma
- 别称:
- IFG
- IFI
- IFN-gamma
- IMD69
- immune interferon
- interferon, gamma
- 基因序列:NCBI_Gene: 3458
- 蛋白序列:UniProtKB: P01579
人 Human IFNG靶点分子功能(预测)
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).
引用文献统计
该产品被引用的文献总数为:169
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