EK180

Human IFN-γ ELISA Kit (人γ干扰素ELISA 试剂盒)

¥1,600.00¥2,650.00

  • 分子靶点:IFNG, IFN-γ
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:50 μL;细胞培养上清:100 μL
  • 灵敏度:0.30 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:83% - 113%

在售SKU:70-EK180-48, 70-EK180-96


描述

商品名

Human IFN-γ ELISA Kit (人γ干扰素ELISA 试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.5% - 7.6%;板间变异系数:3.6%-4.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:50 μL;细胞培养上清:100 μL

灵敏度

0.30 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

83% - 113%

平均回收率

0.95

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗人IFN-γ单克隆抗体的96孔聚苯乙烯酶标板
  • 人IFN-γ冻干标准品
  • IFN-γ 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IFN-γ ELISA Kit (人γ干扰素ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名:IFNG, interferon gamma
  • 基因家族:Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。

人 Human IFNG 分子靶点信息

  • 分子名:IFNG, interferon gamma
  • 别称:
    • IFG
    • IFI
    • IFN-gamma
    • IMD69
    • immune interferon
    • interferon, gamma
  • 基因序列:NCBI_Gene: 3458
  • 蛋白序列:UniProtKB: P01579

人 Human IFNG靶点分子功能(预测)

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

操作步骤


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引用文献

  1. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  2. 血清

    Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

  3. Association Analysis of Polymorphisms in Oas1 with Susceptibility and Severity of Hand, Foot and Mouth Disease 

  4. Inverse Relationship between Toxic Shock Syndrome Toxin-1 Antibodies and Interferon-Γ and Interleukin-6 in Peripheral Blood Mononuclear Cells from Patients with Pediatric Tonsillitis Caused by Staphylococcus Aureus 

  5. Development of T Cells Redirected to Glypican-3 for the Treatment of Hepatocellular Carcinoma 

  6. Aryl Hydrocarbon Receptor Modulates the Expression of Tnf-Α and Il-8 in Human Sebocytes Via the Myd88-P65nf-Κb/P38mapk Signaling Pathways 

  7. A Multistage Subunit Vaccine Effectively Protects Mice against Primary Progressive Tuberculosis, Latency and Reactivation 

  8. Accurate Control of Dual-Receptor-Engineered T Cell Activity through a Bifunctional Anti-Angiogenic Peptide 

  9. Antigen Presentation of the Oct4 and Sox2 Peptides by Cd154-Activated B Lymphocytes Enhances the Killing Effect of Cytotoxic T Lymphocytes on Tumor Stem-Like Cells Derived from Cisplatin-Resistant Lung Cancer Cells 

  10. Microrna-34a Promotes Micb Expression in Hepatocytes 

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