【DESCREPTION】Interleukin 17A (IL-17 or IL-17A), is the founding member of a group of cytokines called the IL-17 family. IL-17 is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation. IL-17 is produced by T-helper cells and is induced by IL-23. IL-17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen’s cellular matrix. IL-17 acts synergistically with tumor necrosis factor and IL-1. IL-17 family has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumour immunity and recently psoriasis and multiple sclerosis.
【PRINCIPLE OF THE ASSAY】This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-17A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-17A present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-17A is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound in the initial step. The color development is stopped and the intensity of the color is measured.
【回收率范围】82 %-120 %
【板间变异系数】4.1 %-4.9 %
【板内变异系数】4.8 %-9.7 %