SuperGold一抗上线，火速来抢！

担心抗体质量不好，不敢用？做出来阳性结果，也觉得是碰运气？联科生物帮您排忧解惑！

MultiSciences SuperGold一抗上线，经过了多种样本和实验方法的验证，保证您实验结果真实有效！这些SuperGold一抗是当下热门研究的抗体，主要包括：AKT1，BAX，Bcl-2，ERK1，ERK2，CD34，Beta-Catenin，EP-CAM等100种抗体。

(一)部分产品应用介绍：1. ab40001（SuperGold Anti-AKT1）
Recommended Dilutions:

WB: 1:2,000 IHC: 1:100-1:200

ICC: 1:100-1:200 FC: 100-1:200

Fig1:
Western blot analysis of Akt1 on differentcell
lysates using anti-Akt1 antibody at 1/2000
dilution.
Positive control:
Lane 1: HepG2 Lane 2: MCF-7 Lane 3: Hela

Fig2:
ICC staining Akt1 inHela cells (red). Cells were
fixed in paraformaldehyde, permeabilised with
0.25% Triton X100/PBS.

Fig3:
Immunohistochemical analysis of
paraffin-embedded human breast carcinoma tissue
using anti-Akt1antibody. Counter stained with hematoxylin.

Fig4:
Flow cytometric analysis of Hela cells with Akt1antibody
at 1/100 dilution (blue) compared with an unlabelled
control (cells without incubation with primary antibody; red).
Goat anti rabbit IgG (FITC) was used as the secondary antibody.

2. ab40005（SuperGold Anti-BAX）
Recommended Dilutions:

WB: 1:500 ICC: 1:200

IHC: 1:200 FC: 1:50

Fig1:
Western blot analysis of Bax on different cell
lysates using anti-BAX antibody at 1/500 dilution.
Positive control:
Lane1: Raji
Lane2: Mouse lymphoid tissue

Fig2:
ICC staining BAX in HepG2 cells (green).
The nuclear counter stain is DAPI (blue).
Cells were fixed in paraformaldehyde,
permeabilised with 0.25% Triton X100/PBS.

Fig3:
Immunohistochemical analysis of
paraffin-embedded human gallbladder tissue
using BAX antibody. Counter stained with
hematoxylin.

Fig4:
Flow cytometric analysis of Hela cells with
vimentin antibody at 1/50 dilution (blue)
compared with an unlabelled control (cells
without incubation with primary antibody;
red). Goat anti mouse IgA (FITC) was used
as the secondary antibody.
3. ab40007（SuperGold Anti-Bcl-2）
Recommended Dilutions:

WB: 1:500 ICC: 1:200

IHC: 1:200 FC: 1:50-1:100

Fig1:
Western blot analysis of Bcl-2 on different
cell lysates using anti-Bcl-2 antibody at 1/500 dilution.
Positive control:
Lane1: HL-60 Lane2: Hela
Lane3: HepG2 Lane 4:A431

Fig2:
ICC staining Bcl-2 in Hela cells (green). Cells were
fixed in paraformaldehyde, permeabilised with 0.25%
Triton X100/PBS.

Fig3:
Immunohistochemical analysis of paraffin-embedded
human colon carcinoma tissue using anti-Bcl-2 rabbit
polyclonal antibody. Counter stained with hematoxylin.

Fig4:
Flow cytometric analysis of Hela cells with Bcl-2 antibody

at 1/50 dilution (blue) compared with an unlabelled control

(cells without incubation with primary antibody; red). Goat

anti rabbit IgG (FITC) was used as the secondary antibody.

4. ab40036（SuperGold Anti-ERK1）
Recommended Dilutions:

WB: 1:500-1:1,000

ICC: 1:200 IHC: 1:200

Fig1:
Western blot analysis of ERK1 on differentcell lysates using
anti- ERK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela Lane2: SW480
Lane3: HCT116 Lane 4: HepG2
Lane5: PC12

Fig2:
ICC staining ofERK1 in LOVO cells (green). Cells were fixed
in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3:

Immunohistochemical analysis of paraffin-embedded human

breast carcinoma tissue using anti-ERK1 antibody. Counter

stained with hematoxylin.

5. ab40037（SuperGold Anti-ERK2）
Recommended Dilutions:

WB: 1:1,000-1:2,000 ICC: 1:200

IHC: 1:200 FC: 1:100-1:200

Fig1: Western blot analysis of ERK2 on differentcell
lysates using anti-ERK2 antibody at 1/2000 dilution.
Positive control:
Lane 1: Jurkat Lane 2:A431 Lane 3: PC12
Lane 4: NIH/3T3 Lane 5: Hela Lane 6: HT-29
Lane 7: MCF-7

Fig2:
ICC staining of ERK2 in A549 cells (green). Cells were fixed
in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3:
Immunohistochemical analysis of paraffin-embedded human breast

carcinoma tissue using anti-ERK2antibody. Counter stained with hematoxylin.

Fig6:
Flow cytometric analysis of Hela cells with SIRT1 antibody at 1/100
dilution (blue) compared with an unlabelled control (cells without
incubation with primary antibody; red). Goat anti rabbit IgG (FITC)
was used as the secondary antibody.

(二) MultiSciences SuperGold抗体清单：