eBioscience T细胞和B细胞活化功能型抗体

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https://www.liankebio.com/article-information_Newsletter-1347.html

作者:eBioscience官网 发布日期:2013-07-31 15:00

评价任何生物系统,最重要的是用抗体、蛋白或者小分子 在细胞和组织水平,以及整个生物体模型中 模仿和/或 抑制相关的通路。

使用这些生物分析工具,使得生命科学研究者得意开发生物系统并检验:

? 通过受体-配体相互作用起始信号的级联放大;

? 诱导细胞活化、分化、迁移和存活;

? 表达特殊的表面和功能表型;

? 蛋白表达样式评估。
eBioscience功能型抗体的优点:
? 具有活化、阻断和中和的功能活性;

? 相比产业标准显著更低的内毒素水平;

? 体内体外应用均不含防腐剂。

T细胞活化抗体

完全活化的T细胞至少需要2个信号。第一信号是TCR捕获抗原递呈细胞通过MHC递呈的肽,第二信号是在T细胞表面表达的共刺激分子与APC上配体结合时产生。这些活化信号一起被传递到T细胞的核内并且产生以下效应:

1) T 细胞的克隆扩增;

2) T细胞表面活化marker的上调;

3) 在其他因素存在时分化为效应性细胞;

4) 诱导细胞毒性和细胞因子分泌;

5) 诱导凋亡。评估T细胞活化的常用方法是通过TCR的竞争性抗体体外刺激T细胞并检测T细胞增殖。

T Cell Activation
Antibodies Clone Cat. No.
Anti-Human CD3 Functional Grade Purified* HIT3a 85-16-0039
Anti-Human CD3 Functional Grade Purified* OKT3 85-16-0037
Anti-Human CD3 Functional Grade Purified SK7 85-16-0036
Anti-Human CD3 Functional Grade Purified UCHT1 85-16-0038
Anti-Human CD28 Functional Grade Purified* CD28.2 85-16-0289
Anti-Human CD28 Functional Grade Purified CD28.6 85-16-0288
Anti-Mouse CD3e Functional Grade Purified* 145-2C11 85-16-0031
Anti-Mouse CD3e Functional Grade Biotin 145-2C11 85-36-0031
Anti-Mouse CD3 Functional Grade Purified 17A2 85-16-0032
Anti-Mouse CD3e Functional Grade Purified eBio500A2 85-16-0033
Anti-Mouse CD28 Functional Grade Purified* 37.51 85-16-0281
Anti-Rat CD3 Functional Grade Purified eBioG4.18 85-16-0030
Anti-Rat CD28 Functional Grade Purified JJ319 85-16-0280
Small Molecules
Cell Stimulation Cocktail (500X) -- 85-00-4970
Cell Stimulation Cocktail (plus protein transport inhibitors)(500X) -- 85-00-4975

B细胞活化

与T细胞活化一样,B细胞的活化同样也需要2个信号。第一信号来自于B细胞受体与其同源的抗原结合。这个信号可以用anti-IgM或IgD抗体来模仿。第二信号来自于CD40或者细胞因子信号与共刺激分子结合。或者,细菌的细胞壁成分,如LPS,以及具有高度重复分子的抗原也可以直接活化B细胞。

B Cell Activation
Antibodies Clone Cat. No.
Anti-Mouse lgD Functional Grade Purified 10.4.22 85-16-5924
Anti-Mouse lgD antiserum polyclonal 85-24-5093
Anti-Mouse lgM μ-chain polyclonal 85-24-5092
Anti-Mouse CD40 Functional Grade Purified 1C10 85-16-0401
Anti-Mouse/Rat CD40 Functional Grade Purified HM40-3 85-16-0402
Anti-Human CD40 Functional Grade Purified 5C3 85-16-0409
Anti-Mouse CD180(RP105) Functional Grade Purified RP/14 85-16-1801
Anti-Human CD180(RP105) Functional Grade Purified MHR73-11 85-16-1809
Recombinant Proteins Cat.No.
Human CD257(BAFF)Recombinant Protein 85-14-8943
Mouse IFN gamma Recombinant Protein 85-14-8311
85-34-8311
Human IFN gamma Recombinant Protein 85-14-8319
85-34-8319
Mouse IL-4 Recombinant Protein 85-14-8041
85-34-8041
Human IL-4 Recombinant Protein 85-14-8049
85-34-8049
Mouse IL-5 Recombinant Protein 85-14-8051
85-34-8051
Mouse TGF-beta 1 Recombinant Protein 85-14-8342
85-34-8342
Human TGF-beta 1 Recombinant Protein 85-14-8349
85-34-8349