论文分享 | 联科凋亡试剂助力巨噬细胞机理研究

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2024年4月1日，中国武汉大学中南医院放射科，精准医学研究所；转化医学中心Maosheng Cheng , Shuang Chen 老师在Nature Communications（IF=16.6）上，在线发表题为“CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer”的论文，此论文使用了联科生物Annexin V-APC/7-AAD Apoptosis Kit 细胞凋亡试剂盒(货号：AP105)

MDSCs (j) and macrophages(k) from control and cKO male mice. Data presented as mean ± SD (n = 6). P valueswere calculated by two-tailed unpaired Student’s t test. l Representative flow cytometry plots of tumor (left) and quantification of percentages of efferocytosis in macrophages of different treatment groups (right). Data was shown as mean ± SD (n = 6).

先天免疫细胞和适应性免疫细胞之间的相互作用对于抗肿瘤免疫反应很重要。然而，肿瘤微环境可能会产生免疫抑制，和肿瘤相关的巨噬细胞正是在这种转变中发挥作用。研究发现，在肿瘤相关巨噬细胞（TAM）上表达的CD276在减少对肿瘤的免疫反应中发挥作用。实验中使用N-丁基-N-（4-羟基丁基）亚硝胺诱导的BLCA雄性小鼠肿瘤模型，发现TAMs中CD276的遗传消融阻断了胞饮作用并增强了TAM的主要组织相容性复合物II类（MHCII）的表达。这反过来又增加了肿瘤的CD4 +和细胞毒性CD8 + T细胞浸润。结合单细胞RNA测序和功能实验，CD276激活溶酶体信号通路和转录因子JUN调节AXL和MerTK的表达，从而增强TAMs的胞吐作用。综上所述，该研究支持CD276在TAM的胞饮作用中的作用，这可能是联合免疫治疗的潜在靶点。

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参考文献：Cheng, Mao sheng et al. CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer. Nature communications vol. 15,1 2818. 1 Apr. 2024.

原文链接：https://www.nature.com/articles/s41467-024-46735-5#Sec2