Microrna-21 Antisense Oligonucleotide Improves the Sensitivity of A375 Human Melanoma Cell to Cisplatin: An in Vitro Study

  • 类型:
  • 作者:Zhang, H. L., Si, L. B., Zeng, A., Long, F., Qi, Z., Zhao, R. & Bai, M.
  • 期刊:Journal of cellular biochemistry 119, 3129-3141 (2018)
  • 阅读原文

This study explored Cisplatin resistance effect of microRNA-21 (miR-21) antisense oligonucleotide (AS-ODN) in human melanoma A375 cell. AS-ODN was transfected in melanoma A375 cells and Cisplatin-resistant cell line A375/CDDP, and divided into the AS-ODN, nonsense oligonucleotide (NS-ODN) and normal groups. Cell ultrastructure changes were observed through transmission electron microscope. MiR-21 AS-ODN could be tested cell growth effect in different time periods by trypan blue exclusion. MiR-21 mRNA expression change was detected by quantitative fluorescence PCR. Cell apoptosis, cycle distribution and miR-21 AS-ODN effect on proliferation and Cisplatin sensitivity were tested by flow cytometry, MTT assay, TUNEL, and Clonogenic assay. Cell apoptosis was observed after transfection 24?h with the AS-ODN group, while the NS-ODN and normal group cells had no apoptotic symptoms; Compared with the normal group, the AS-ODN group began to show obvious cell growth inhibition effect after transfection 24?h lasting 72?h (all P??0.05). miR-21 mRNA expression in the AS-ODN group was obviously decreased with rising apoptosis rate (all P??0.05). MiR-21 AS-ODN could remarkably increase A375 cell and A375/CDDP cell sensitivity to Cisplatin (P?文章引用产品