CCS012

Cell Cycle Staining Kit 细胞周期检测试剂盒

¥600.00

在售SKU:70-CCS012, 细胞周期,周期试剂盒,细胞周期试剂盒,周期,细胞周期检测,试剂盒


描述

文章目录[隐藏]

产品简介

碘化丙啶(Propidium iodide, PI)可插入碱基之间而与DNA结合,其对碱基几乎没有序列偏好,每4 - 5个碱基对可结合一个染料分子。PI也能与RNA结合,需要用核酸酶处理来区分RNA和DNA染色。一旦染料与核酸结合,其荧光会增强20 - 30倍。虽然其摩尔吸收率(吸光系数)相对较低,PI仍然具有较大的stokes位移,从而在滤光片选择恰当的情况下,仍能同时检测细胞核DNA和荧光标记的抗体。PI可用于荧光显微镜、激光共聚焦、流式细胞仪和荧光计。

PI为膜不通透性,被活细胞排斥,可用于鉴定死细胞和多色荧光复染。下面提供的操作步骤与很多细胞标记技术兼容,如直接或间接抗体检测技术、mRNA原位杂交、特异性的细胞结构染料等。在组织染色中可能要作修改。

本试剂盒包含50 ml DNA染色液和500 μl破膜剂,足够用于50次检测。

产品特点

  • 即用型,操作简单
  • 活细胞/固定细胞检测
  • 常备现货

引用文献

文章目录[隐藏]


  1. 人胰腺癌细胞

    RELA-induced MiR-21 Exerts Oncogenic Effects on PDAC via Targeting of ARHGAP24 

  2. 膀胱成纤维细胞

    Involvement of Activation of Mitogen-Activated Protein Kinase (Mapk)/Extracellular Signal-Regulated Kinase (Erk) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias 

  3. TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferation 

  4. Movement Disorder and Neurotoxicity Induced by Chronic Exposure to Microcystin-LR in Mice 

  5. Downregulation of Usp18 Inhibits Growth and Induces Apoptosis in Hepatitis B Virus-Related Hepatocellular Carcinoma Cells by Suppressing Bcl2l1 

  6. Therapeutic Potential of Transplanted Placental Mesenchymal Stem Cells in Treating Chinese Miniature Pigs with Acute Liver Failure 

  7. Effects of 2-Aryl-1-Cyano-1,2,3,4-Tetrohydroisoquinolines on Apoptosis Induction Mechanism in Nb4 and Mkn-45 Cells 

  8. Mir-425-5p Inhibits Differentiation and Proliferation in Porcine Intramuscular Preadipocytes 

  9. Knockdown of Eukaryotic Translation Initiation Factor?4e Suppresses Cell Growth and Invasion, and Induces Apoptosis and Cell Cycle Arrest in a Human Lung Adenocarcinoma Cell Line 

  10. Akt3 Is a Target of Mir-29c-3p and Serves an Important Function in the Pathogenesis of Congenital Heart Disease 

  11. Overexpression of Hsd17b4 Exerts Tumor Suppressive Function in Adrenocortical Carcinoma and Is Not Associated with Hormone Excess 

  12. Lysophosphatidylcholine Acyltransferase 1 Upregulation and Concomitant Phospholipid Alterations in Clear Cell Renal Cell Carcinoma 

  13. Downregulation of Ubc9 Promotes Apoptosis of Activated Human Lx-2 Hepatic Stellate Cells by Suppressing the Canonical Nf-Κb Signaling Pathway 

  14. Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in Jb6 Cells May Be through Down-Regulation of the Mapk Signaling Pathways 

  15. Comparison in the Biological Characteristics between Primary Cultured Sensory and Motor Schwann Cells 

  16. High Expression of G-Protein Signaling Modulator 2 in Hepatocellular Carcinoma Facilitates Tumor Growth and Metastasis by Activating the Pi3k/Akt Signaling Pathway 

  17. The Aldehyde Group of Gossypol Induces Mitochondrial Apoptosis Via Ros-Sirt1-P53-Puma Pathway in Male Germline Stem Cell 

  18. Exendin-4 Modifies Adipogenesis of Human Adipose-Derived Stromal Cells Isolated from Omentum through Multiple Mechanisms 

  19. Mir-375 Controls Porcine Pancreatic Stem Cell Fate by Targeting 3-Phosphoinositide-Dependent Protein Kinase-1 (Pdk1) 

  20. Suppressed Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties Mediate the Anti-Cancer Effects of Ethyl Pyruvate Via Regulation of the Akt/Nuclear Factor-Κb Pathway in Prostate Cancer Cells 

  21. Immune Modulation of a Lipid-Soluble Extract of Pinellia Pedatisecta Schott in the Tumor Microenvironment of an Hpv(+) Tumor-Burdened Mouse Model 

  22. Ripk1 Inhibition Enhances Pirarubicin Cytotoxic Efficacy through Akt-P21-Dependent Pathway in Hepatocellular Carcinoma 

  23. Mir-19-5p Enhances Tumorigenesis in Human Colorectal Cancer Cells by Targeting Tspyl5 

  24. Thromboxane A2 Exerts Promoting Effects on Cell Proliferation through Mediating Cyclooxygenase-2 Signal in Lung Adenocarcinoma Cells 

  25. Mechanism of All-Transretinoic Acid Increasing Retinoblastoma Sensitivity to Vincristine 

  26. Col11a1 Is Overexpressed in Gastric Cancer Tissues and Regulates Proliferation, Migration and Invasion of Hgc-27 Gastric Cancer Cells In?vitro 

  27. Regulatory Effects of Δfosb on Proliferation and Apoptosis of Mcf-7 Breast Cancer Cells 

  28. Circfut10 Reduces Proliferation and Facilitates Differentiation of Myoblasts by Sponging Mir-133a 

  29. Inhibition of Ezh2 Via Activation of Sapk/Jnk and Reduction of P65 and Dnmt1 as a Novel Mechanism in Inhibition of Human Lung Cancer Cells by Polyphyllin I 

  30. Eif2s3y Suppresses the Pluripotency State and Promotes the Proliferation of Mouse Embryonic Stem Cells 

  31. Fenofibrate Induces Apoptosis of Triple-Negative Breast Cancer Cells Via Activation of Nf-Κb Pathway 

  32. Expression and Function of the Actin-Severing Protein Adseverin in the Proliferation, Migration, and Differentiation of Dental Pulp Cells 

  33. DNA Damage Induced by Human Cd40 Ligand Mutant Promotes Senescence and Induces Demethylation of Gata4 in Lung Cancer 

  34. Decreased Long Non-Coding Rna Mtm Contributes to Gastric Cancer Cell Migration and Invasion Via Modulating Mt1f 

  35. Dehydroepiandrosterone Inhibits Cell Proliferation and Improves Viability by Regulating S Phase and Mitochondrial Permeability in Primary Rat Leydig Cells 

  36. Ras-Association Domain Family 10 Acts as a Novel Tumor Suppressor through Modulating Mmp2 in Hepatocarcinoma 

  37. Tumor Suppressor Bromodomain-Containing Protein 7 Cooperates with Smads to Promote Transforming Growth Factor-Β Responses 

  38. Mir-122 Modification Enhances the Therapeutic Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cells against Liver Fibrosis 

  39. Exosomes Derived from Mir-122-Modified Adipose Tissue-Derived Mscs Increase Chemosensitivity of Hepatocellular Carcinoma 

  40. The Inhibition of 2,3-Dichloro-1-Propanol on T Cell in Vitro and in Vivo 

  41. Mirna-186 Inhibits Prostate Cancer Cell Proliferation and Tumor Growth by Targeting Yy1 and Cdk6 

  42. Profilin 1 Induces Drug Resistance through Beclin1 Complex-Mediated Autophagy in Multiple Myeloma 

  43. Inhibition of C5a Prevents Il-1β-Induced Alternations in Rat Synoviocytes in Vitro 

  44. Cbx3 Predicts an Unfavorable Prognosis and Promotes Tumorigenesis in Osteosarcoma 

  45. Alternative Splicing of Spleen Tyrosine Kinase Differentially Regulates Colorectal Cancer Progression 

  46. The Membrane Complement Regulatory Protein Cd59 Promotes Tumor Growth and Predicts Poor Prognosis in Breast Cancer 

  47. Decreased Expression of Calpain-9 Predicts Unfavorable Prognosis in Patients with Gastric Cancer 

  48. Erk1/2 Inhibition Enhances Apoptosis Induced by Jak2 Silencing in Human Gastric Cancer Sgc7901 Cells 

  49. Mir-30b-5p Acts as a Tumor Suppressor, Repressing Cell Proliferation and Cell Cycle in Human Hepatocellular Carcinoma 

  50. Prolyl Hydroxylases Positively Regulated Lps-Induced Inflammation in Human Gingival Fibroblasts Via Tlr4/Myd88-Mediated Akt/Nf-Κb and Mapk Pathways 

  51. Effect-Enhancing and Toxicity-Reducing Activity of Usnic Acid in Ascitic Tumor-Bearing Mice Treated with Bleomycin 

  52. Effects of the Calcium-Activated Chloride Channel Inhibitors T16ainh-A01 and Caccinh-A01 on Cardiac Fibroblast Function 

  53. Inhibition of Anaphase-Promoting Complex by Silence Apc/C(Cdh1) to Enhance Radiosensitivity of Nasopharyngeal Carcinoma Cells 

  54. The Inhibitory Effect of 3 Β -Hydroxy-12-Oleanen-27-Oic Acid on Growth and Motility of Human Hepatoma Hepg2 Cells through Jnk and Akt Signaling Pathway 

  55. Knockdown of Parp6 or Survivin Promotes Cell Apoptosis and Inhibits Cell Invasion of Colorectal Adenocarcinoma Cells 

  56. Adenovirus Type 12 E1b 55-Kilodalton Oncoprotein Promotes P53-Mediated Apoptotic Response of Ovarian Cancer to Cisplatin 

  57. Mir-208b Regulates Cell Cycle and Promotes Skeletal Muscle Cell Proliferation by Targeting Cdkn1a 

  58. Oncogene Rpa1 Promotes Proliferation of Hepatocellular Carcinoma Via Cdk4/Cyclin-D Pathway 

  59. Curcusone C Induces Telomeric DNA-Damage Response in Cancer Cells through Inhibition of Telomeric Repeat Factor 2 

  60. Characterization of the Release and Biological Significance of Cell-Free DNA from Breast Cancer Cell Lines 

  61. Mir-363-3p Inhibits Tumor Growth by Targeting Pcna in Lung Adenocarcinoma 

  62. Anti-Angiogenic and Antitumor Activities of Huaier Aqueous Extract 

  63. Downregulation of Toll-Like Receptor 4 Induces Suppressive Effects on Hepatitis B Virus-Related Hepatocellular Carcinoma Via Erk1/2 Signaling 

  64. Up-Regulation of Cdk16 by Multiple Mechanisms in Hepatocellular Carcinoma Promotes Tumor Progression 

  65. Myocyte Enhancer Factor 2a Promotes Proliferation and Its Inhibition Attenuates Myogenic Differentiation Via Myozenin 2 in Bovine Skeletal Muscle Myoblast 

  66. Circular Rna Profiling Reveals an Abundant Circlmo7 That Regulates Myoblasts Differentiation and Survival by Sponging Mir-378a-3p 

  67. Matrine Induces Akt/Mtor Signalling Inhibition-Mediated Autophagy and Apoptosis in Acute Myeloid Leukaemia Cells 

  68. 0404 Inhibits Hepatocellular Carcinoma through a P53/Mir-34a/Sirt1 Positive Feedback Loop 

  69. Crosstalk of Nf-Κb/P65 and Lncrna Hotair-Mediated Repression of Muc1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1-Enzalutamide Combination Treatment 

  70. Butyrate Upregulates the Tlr4 Expression and the Phosphorylation of Mapks and Nk-Κb in Colon Cancer Cell in Vitro 

  71. Promoting Tumorigenesis in Nasopharyngeal Carcinoma, Nedd8 Serves as a Potential Theranostic Target 

  72. Genetic Inhibition of Vascular Endothelial Growth Factor Receptor-1 Significantly Inhibits the Migration and Proliferation of Leukemia Cells and Increases Their Sensitivity to Chemotherapy 

  73. The Oncogenic Role of Col23a1 in Clear Cell Renal Cell Carcinoma 

  74. Gosb Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells 

  75. Sodium Butyrate Inhibits Colorectal Cancer Cell Migration by Downregulating Bmi-1 through Enhanced Mir-200c Expression 

  76. Modulation of Igf2bp1 by Long Non-Coding Rna Hcg11 Suppresses Apoptosis of Hepatocellular Carcinoma Cells Via Mapk Signaling Transduction 

  77. Inhibitory Effects of Traditional Chinese Herbal Medicine Tanreqing Injection on Proliferation of Leukemia Cells in Vitro and the Potential Mechanisms 

  78. Ras-Related Associated with Diabetes Gene Acts as a Suppressor and Inhibits Warburg Effect in Hepatocellular Carcinoma 

  79. Oridonin Inhibits Oral Cancer Growth and Pi3k/Akt Signaling Pathway 

  80. Liver-Enriched Activator Protein 1 as an Isoform of Ccaat/Enhancer-Binding Protein Beta Suppresses Stem Cell Features of Hepatocellular Carcinoma 

  81. Cuprous Oxide Nanoparticles Trigger Er Stress-Induced Apoptosis by Regulating Copper Trafficking and Overcoming Resistance to Sunitinib Therapy in Renal Cancer 

  82. Anticancer Effect of Quinacrine on Diffuse Large B?Cell Lymphoma Via Inhibition of Msi2?Numb Signaling Pathway 

  83. Increased Invasiveness of Osteosarcoma Mesenchymal Stem Cells Induced by Bone-Morphogenetic Protein-2 

  84. Long Noncoding Rna Rp11-766n7.4 Functions as a Tumor Suppressor by Regulating Epithelial-Mesenchymal Transition in Esophageal Squamous Cell Carcinoma 

  85. Equol Inhibits Proliferation of Human Gastric Carcinoma Cells Via Modulating Akt Pathway 

  86. Changes of Number of Cells Expressing Proliferation and Progenitor Cell Markers with Age in Rabbit Intervertebral Discs 

  87. Overexpression of Zwint Predicts Poor Prognosis and Promotes the Proliferation of Hepatocellular Carcinoma by Regulating Cell-Cycle-Related Proteins 

  88. Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma 

  89. Cancer-Associated Morc2-Mutant M276i Regulates an Hnrnpm-Mediated Cd44 Splicing Switch to Promote Invasion and Metastasis in Triple-Negative Breast Cancer 

  90. Preservation Media, Durations and Cell Concentrations of Short-Term Storage Affect Key Features of Human Adipose-Derived Mesenchymal Stem Cells for Therapeutic Application 

  91. Microrna-21 Antisense Oligonucleotide Improves the Sensitivity of A375 Human Melanoma Cell to Cisplatin: An in Vitro Study 

  92. Ad-1, a Novel Ginsenoside Derivative, Shows Anti-Lung Cancer Activity Via Activation of P38 Mapk Pathway and Generation of Reactive Oxygen Species 

  93. Hydrogen Alleviates Cellular Senescence Via Regulation of Ros/P53/P21 Pathway in Bone Marrow-Derived Mesenchymal Stem Cells in Vivo 

  94. Candida Glabrata Induced Infection of Rat Tracheal Epithelial Cells Is Mediated by Tlr-2 Induced Activation of Nf-Κb 

  95. Interaction of Quindoline Derivative with Telomeric Repeat-Containing Rna Induces Telomeric DNA-Damage Response in Cancer Cells through Inhibition of Telomeric Repeat Factor 2 

  96. Loss of Olfm4 Promotes Tumor Migration through Inducing Interleukin-8 Expression and Predicts Lymph Node Metastasis in Early Gastric Cancer 

  97. In Vitro Effects of Nerve Growth Factor on Cardiac Fibroblasts Proliferation, Cell Cycle, Migration, and Myofibroblast Transformation 

  98. Upregulation of Tyrosine Kinase Fyn in Human Thyroid Carcinoma: Role in Modulating Tumor Cell Proliferation, Invasion, and Migration 

  99. Downregulation of Cohesin Loading Factor Nipped-B-Like Protein (Nipbl) Induces Cell Cycle Arrest, Apoptosis, and Autophagy of Breast Cancer Cell Lines 

  100. 2-Methoxyestradiol Inhibits Hypoxia-Induced Scleroderma Fibroblast Collagen Synthesis by Phosphatidylinositol 3-Kinase/Akt/Mtor Signalling 

  101. Mir-196b/Mir-1290 Participate in the Antitumor Effect of Resveratrol Via Regulation of Igfbp3 Expression in Acute Lymphoblastic Leukemia 

  102. Microrna-141-3p Promotes Glioma Cell Growth and Temozolomide Resistance by Directly Targeting P53 

  103. Pifithrin-Μ Is Efficacious against Non-Small Cell Lung Cancer Via Inhibition of Heat Shock Protein 70 

  104. Alpha1-Act Functions as a Tumour Suppressor in Hepatocellular Carcinoma by Inhibiting the Pi3k/Akt/Mtor Signalling Pathway Via Activation of Pten 

  105. Loss of Angiotensin-Converting Enzyme 2 Promotes Growth of Gallbladder Cancer 

  106. Downregulated Long Non-Coding Rna Trpm2-as Inhibits Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Via Activation of P53- P66shc Pathway 

  107. Long Non-Coding Rna Xist Functions as an Oncogene in Human Colorectal Cancer by Targeting Mir-132-3p 

  108. Angiopoietin-1 Facilitates Recovery of Hematopoiesis in Radiated Mice 

  109. The Malignancy of Mir-18a in Human Glioblastoma Via Directly Targeting Cbx7 

  110. Microrna-625 Inhibits the Proliferation and Increases the Chemosensitivity of Glioma by Directly Targeting Akt2 

  111. Microrna-1301 Inhibits Proliferation of Human Glioma Cells by Directly Targeting N-Ras 

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检测步骤

一、可当天检测的活细胞样本

  1. 收集2 × 105- 1 × 106个细胞,离心弃上清。用PBS洗涤一次,离心弃上清。
  2. 加入1 mL DNA Staining Solution和10 μL Permeabilization Solution,涡旋振荡5 - 10秒混匀。室温避光孵育30分钟。
  3. 选择最低上样速度,在流式细胞仪上进行检测。如需在荧光显微镜下观察,则离心细胞,弃上清,加入新鲜缓冲液重悬。滴加1滴细胞悬液至载玻片,盖上盖玻片后使用合适的滤光片进行观察。

二、当天无法检测的活细胞样本

  1. 按照下述方法固定,或其他合适的方法固定
    • 收集2 × 105- 1 × 106个细胞,离心弃上清。轻弹管壁,使沉淀重悬在残余的液体中,加入1 mL 室温下的PBS。
    • 将细胞缓慢加入至3 mL无水乙醇(-20℃预冷)中,边加边高速搅拌。-20℃固定过夜,可保存数月。
  2. 检测当天,将固定细胞离心,弃去乙醇,轻弹管壁使沉淀松散,加入2 - 5 mL 室温下的PBS,放置15分钟使细胞再次水化。离心,弃上清。
  3. 加入1 ml DNA Staining Solution,涡旋振荡5 - 10秒混匀。室温避光孵育30分钟。
  4. 选择最低上样速度,在流式细胞仪上进行检测。如需在荧光显微镜下观察,则离心细胞,弃上清,加入新鲜缓冲液重悬。滴加1滴细胞悬液至载玻片,盖上盖玻片后使用合适的滤光片进行观察。

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