小科教您选二抗

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https://www.liankebio.com/article-information_Newsletter-63.html

作者:联科生物 发布日期:2016-05-13 17:35

小科教您选二抗
二抗在IF、IHC、WB、ELISA、FC、EMSA、IP和CHIP等多种研究应用中起到重要作用。正确选择二抗可以增加信号检测,减少背景和非特异染色,优化您的实验结果。

如何选择二抗?
1. 根据一抗的种属来源:Goat anti Mouse IgG(H+L), HRP

2. 根据一抗的类别亚型:

Goat anti Mouse IgG(H+L), FITC

Goat Anti Rabbit IgG(γ), PE

Goat Anti Mouse IgM, FITC

3. 根据实验检测方法:荧光、化学发光、显色?

Goat anti Mouse IgG(H+L), HRP

Goat anti Mouse IgG(H+L), FITC

4. 是否需要F(ab’)2或F(ab)片段抗体?

DyLight488 F[ab’]2 of goat anti-rabbit IgG [H+L] 您知道吗?
? Pre-adsorbed antibodies 可以消除不必要的种属交叉反应,抗体片段反应或细胞组织样本的非特异染色。

? F(ab’)2和F(ab)片段二抗可以消除Fc受体的非特异性染色。

? 亲和纯化抗体增加特异染色,降低背景,灵敏度高,批间一致。

联科生物提供多种优质二抗,欢迎您来订购!

我们的优势:

? 性价比高(1:5000-1:100000)

? -20℃保存为液体(不会反复冻融)

? 无需分装,使用方便
一、 酶标二抗

目录号 产品 规格 价格
GAM007[1-6] Goat Anti-Mouse IgG(H+L) HRP 100μl 150
GAM0072 Goat Anti-Mouse IgG(H+L) HRP 200μl 270
GAR007[2-6,7] Goat Anti-Rabbit IgG(H+L) HRP 100μl 150
GAR0072 Goat Anti-Rabbit IgG(H+L) HRP 200μl 270
RAG007[5,8] Rabbit Anti-Goat IgG(H+L) HRP 100μl 180
RAG0072 Rabbit Anti-Goat IgG(H+L) HRP 200μl 320
GRT007 Goat Anti-Rat IgG(H+L) HRP 100μl 240

小科提醒:我们的稀释比极高,价格也很优惠哦!
二、 荧光二抗特点:
? ? 亮度更高-保证出色的灵敏性
? ? 光稳定性更好-对光漂白有超强抵抗能力
? ? 更高的光学耐受性(抗淬灭性)
? ? 仪器兼容性好-发射光谱与普通荧光设备的主要输出波长匹配
? ? 性价比高

标记物(Ex/Em) 山羊抗小鼠 山羊抗兔 兔抗山羊
产品目录号 规格 价格 产品目录号 规格 价格 产品目录号 规格 价格
FITC(492/520) GAM001 100μl 300 GAR001 100μl 300 RAG001 100μl 360
PE(496/578) GAM0041[9] 100μl 480 GAR0042 100μl 480
DyLight488(493/518) GAM4882[5] 100μl 500 GAR4882[5] 100μl 500 RAG4882 100μl 600
DyLight488 F(ab’)2 GAM4881[10] 50μl 500 GAR4881[10] 50μl 500 RAG4881 50μl 600
DyLight549(555/568) GAM5492[5,11] 100μl 500 GAR5492[5] 100μl 500 RAG5492 100μl 600
DyLight594(591/616) GAM5942 100μl 500 GAR5942 100μl 500 RAG5942 100μl 600
DyLight649(652/670) GAM6492 100μl 500 GAR6492 100μl 500

小科提醒:DyLight 488替代FITC/Cy2/Alexa Fluor 488;
DyLight 649 替代APC/Cy5/Alexa Fluor 647;
DyLight 549和DyLight 594不可做FCM!

图:用DyLight405、DyLight488、DyLight649分别标记的二抗与相应一抗反应,
对小鼠视网膜组织染色的共聚焦结果图.加州大学圣巴巴拉分校

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