论文分享 | 29.4分!一种用于入侵肿瘤细胞的细菌生物杂交体

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2024年4月2日,武汉大学张先正教授团队在Advanced Materials ( IF 29.4) 上发表了题为:Bacterial Biohybrids for Invasion of Tumor Cells Promote Antigen Cross-Presentation Through Gap Junction 研究论文。此论文使用了联科生物2种ELISA检测试剂盒对小鼠TNF-α和IFN-γ蛋白进行了定量检测:Mouse TNF-α ELISA Kit(货号:EK282)MouseIFN-γELISA Kit(货号:EK280)。

图1研究机制图

由于肿瘤携带的细菌与宿主细胞在细胞组成和代谢行为上的固有差异,这些细菌可以有选择地影响肿瘤的免疫环境。然而,内源性细胞内细菌如何影响肿瘤细胞与抗原呈递细胞(APCs)之间的抗原呈递过程的机制尚不完全清楚。

本文研究了减毒沙门氏菌VNP20009(VNP)侵入肿瘤细胞的行为,并尝试通过修改VNP表面上的正电聚合物来调节这种行为。

研究发现

①非毒性的壳聚糖寡糖(COS)修饰的VNP (VNP@COS)增强了VNP感染肿瘤细胞的能力,从而增强了肿瘤细胞与APCs之间的缝隙连接的形成。

②基于此基础,设计了一种细菌生物混合物,通过细胞内细菌诱导的缝隙连接促进原位抗原交叉呈递。

③这种细菌生物混合物还通过与VNP@COS结合的Mdivi-1增强了肿瘤细胞表面主要组织相容性复合体I类分子的表达。

④这种战略性整合有助于增强肿瘤抗原的免疫原性暴露,同时保留了T细胞的细胞毒性作用。提出了一种可以精确控制微生物在肿瘤内的功能和局部效果的策略。

联科ELISA试剂盒助力科研

货号:EK282

产品名:Mouse TNF-a ELISA Kit检测试剂盒

部分产品引用文献:

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2.(IF=20.8)Shi Y, Lu Y, Qin B, et al. Antigen transfer from non-APCs to APCs impacts the efficacy and safety of protein-and mRNA-based vaccines[J]. Nano Today, 2021, 41: 101326.

3.(IF=19.9)Shi Y, Lou Z, Liu Y, et al. Cell Communication‐Mediated Selective Drug Delivery for Epitope‐Specific Deletion of Alloreactive T Cells in Organ Transplant[J]. Advanced Functional Materials, 2023, 33(52): 2304823.

4.(IF=18.0)Liu Y, Lu Y, Ning B, et al. Intravenous delivery of living listeria monocytogenes elicits gasdmermin-dependent tumor pyroptosis and motivates anti-tumor immune response[J]. ACS nano, 2022, 16(3): 4102-4115.

5.(IF=17.5)Chen H, Zhou M, Zeng Y, et al. Biomimetic lipopolysaccharide‐free bacterial outer membrane‐functionalized nanoparticles for brain‐targeted drug delivery[J]. Advanced Science, 2022, 9(16): 2105854.

货号:EK280

产品名:Mouse IFN-gamma ELISA Kit检测试剂盒

部分产品引用文献:

1.(IF=54.9) Zhou Y, Kong D, Wang X, et al. A small and highly sensitive red/far-red optogenetic switch for applications in mammals[J]. Nature Biotechnology, 2022, 40(2): 262-272.

2.(IF 32.1) Bai P, Liu Y, Xue S, et al. A fully human transgene switch to regulate therapeutic protein production by cooling sensation[J]. Nature medicine, 2019, 25(8): 1266-1273.

3.(IF 19.9) Shi Y, Lou Z, Liu Y, et al. Cell Communication‐Mediated Selective Drug Delivery for Epitope‐Specific Deletion of Alloreactive T Cells in Organ Transplant[J]. Advanced Functional Materials, 2023, 33(52): 2304823.

4.(IF 18.0) Zhang J, Wang S, Guo X, et al. Arginine supplementation targeting tumor-killing immune cells reconstructs the tumor microenvironment and enhances the antitumor immune response[J]. ACS nano, 2022, 16(8): 12964-12978.

5.(IF 17.7) Locally organised and activated Fth1hi neutrophils aggravate inflammation of acute lung injury in an IL-10-dependent manner

参考文献:Li W, Chen QW, Fan JX, Han ZY, Song WF, Zeng X, Zhang XZ. Bacterial Biohybrids for Invasion of Tumor Cells Promote Antigen Cross-Presentation Through Gap Junction. Adv Mater. 2024 Apr 2:e2402532.

原文地址:https://pubmed.ncbi.nlm.nih.gov/38563503/