一、细胞凋亡检测
凋亡检测试剂盒
| 目录号 |
产品名称 |
试剂盒组分 |
规格 |
| AP101-30 |
Annexin V/PI apoptosis kit |
|
Annexin V FITC |
|
PI |
|
5X Binding buffer |
|
30T |
| AP101-60 |
Annexin V/PI apoptosis kit |
60T |
| AP101-100 |
Annexin V/PI apoptosis kit |
100T |
 |
【试剂盒优点】⇒ 操作简便，分群清晰【实验步骤】 ⇒ 收集细胞并重悬于Binding Buffer(结合液)中⇒ 加荧光标记的Annexin V和PI⇒ 室温、避光孵育5分钟⇒ 流式细胞仪检测结果 |
单独提供试剂盒组分
| 目录号 |
产品名称 |
规格 |
| AP101-30-AVF |
Annexin V FITC |
30T |
| AP101-60-AVF |
Annexin V FITC |
60T |
| AP101-100-AVF |
Annexin V FITC |
100T |
| AP101-100-BB |
5X Binding buffer |
15ml |
| AP101-100-PI |
PI solution(For apoptosis detection with Annexin V) |
100T |
| AP101-30-PI |
PI solution(For apoptosis detection with Annexin V) |
30T |
| AP101-60-PI |
PI solution(For apoptosis detection with Annexin V) |
60T |
其他凋亡相关产品
1、其他标记AnnexinV检测试剂盒
| AP104-30 |
Annexin V-PE/7-AAD apoptosis kit适用于检测含GFP或其他绿色荧光的细胞 |
30T |
| AP104-60 |
60T |
| AP104-100 |
100T |
| AP105-30 |
Annexin V-APC/7-AAD apoptosis kit适用于多色流式搭配 |
30T |
| AP105-60 |
60T |
| AP105-100 |
100T |
2、细胞凋亡线粒体检测试剂盒
| 目录号 |
产品名称 |
规格 |
| MJ101 |
Mitochondria Staining Kit(JC-1) |
125T |
 |
【实验步骤】1、收集细胞于MitoCapture溶液 (含JC-1)中2、37oC孵育15-20分钟3、离心、去上清4、重悬于孵育缓冲液中5、流式分析: FL1:凋亡细胞；FL2:正常细胞 |
| 喜树碱(CPT)诱导HL-60细胞凋亡3小时后用JC-1检测线粒体膜电位的改变。可见凋亡细胞红色荧光降低。 |
| 【单独提供试剂盒组分】 |
|
| 目录号 |
产品名称 |
| J200 |
JC-1（2mM） |
| C050 |
CCCP（50mM） |
| S1001 |
Staining buffer |
|
| 3、阳性对照——补偿调节的好帮手 |
阳性对照处理后的AV-FITC单染 |
| 目录号 |
产品名称 |
规格 |
| AP-30-PCS |
Apoptosis Positive Control Solution |
5ml |
| AP-60-PCS |
Apoptosis Positive Control Solution |
10ml |
| AP-100-PCS |
Apoptosis Positive Control Solution |
15ml |
|
 |
二、细胞周期检测试剂
特点：
⇒ 适用范围广——活细胞和固定细胞
⇒ 操作简单——收集重悬的一步法检测
⇒ 结果稳定可靠——分期明显，CV值低
|
（A）活细胞离心收集细胞，洗一遍，弃上清
加1ml Staining buffer（A+B）
室温、避光孵育30分钟
流式细胞仪检测结果 |
（B）固定细胞小心固定细胞，-20度贮存
离心收集细胞，洗一遍，弃上清
加1mlStaining buffer（A）
室温、避光孵育30分钟
流式细胞仪检测结果 |
 |
| 目录号 |
名称 |
试剂盒组分 |
适用范围 |
| CCS01 |
Cell cycle staining buffer |
staining buffer |
一步法活细胞检测 |
| CCS012 |
Cell cycle staining Kit |
staining buffer （A)Permeabilization solution (B) |
活细胞/固定细胞检测 |
| CCS013 |
The Apo-peak DNAanalysis kit |
staining buffer （A)Permeabilization solution (B) DNA extration buffer (C) |
活细胞/固定细胞/凋亡峰检测 |
三、流式抗体和染料
人流式抗体
| 目录号 |
产品名称(简述) |
规格 |
| AH0305-100 |
Anti-Human CD3,APC |
100T |
| AH0305-50 |
Anti-Human CD3,APC |
50T |
| AH0401-100 |
Anti-Human CD4,FITC |
100T |
| AH0401-50 |
Anti-Human CD4,FITC |
50T |
| AH0804-100 |
Anti-Human CD8,PE |
100T |
| AH0804-50 |
Anti-Human CD8,PE |
50T |
小鼠流式抗体
| 目录号 |
产品 |
规格 |
| EM0301-50 |
Mouse CD3 FITC |
50T |
| EM0301-100 |
Mouse CD3 FITC |
100T |
| EM0301-200 |
Mouse CD3 FITC |
200T |
| EM0306-50 |
Mouse CD3 PE-Cy5 |
50T |
| EM0306-100 |
Mouse CD3 PE-Cy5 |
100T |
| EM0306-200 |
Mouse CD3 PE-Cy5 |
200T |
| BM0401-50 |
Mouse CD4 FITC |
50T |
| BM0401-100 |
Mouse CD4 FITC |
100T |
| BM0404-100 |
Mouse CD4 PE |
100T |
| EM0801-100 |
Mouse CD8a FITC |
100T |
| EM0801-50 |
Mouse CD8a FITC |
50T |
| EM0804-100 |
Mouse CD8a PE |
100T |
| EM0804-50 |
Mouse CD8a PE |
50T |
| EM49b04-100 |
Mouse pan-NK cells(CD49b) PE |
100T |
| EM49b04-50 |
Mouse pan-NK cells(CD49b) PE |
50T |
| EMNK04-100 |
Mouse NK1.1 PE |
100T |
| EMNK04-50 |
Mouse NK1.1 PE |
50T |
| EM45R01-100 |
Mouse CD45R (B cells) FITC |
100T |
| EM45R01-50 |
Mouse CD45R (B cells) FITC |
50T |
| EM6901-20 |
Mouse CD69 FITC |
20T |
| EM6904-20 |
Mouse CD69 PE(H1.2F3,Hamster IgG) |
20T |
| EM6901-50 |
Mouse CD69 FITC |
50T |
| EM6904-50 |
Mouse CD69 PE(H1.2F4,Hamster IgG) |
50T |
大鼠流式抗体
| 目录号 |
产品 |
规格 |
| ER0301-100 |
Rat CD3 FITC |
100T |
| ER0301-200 |
Rat CD3 FITC |
200T |
| ER0301-50 |
Rat CD3 FITC |
50T |
| ER0404-100 |
Rat CD4 PE |
100T |
| ER0404-50 |
Rat CD4 PE |
50T |
| ER0405-100 |
Rat CD4 APC |
100T |
| ER0405-50 |
Rat CD4 APC |
50T |
| ER0804-100 |
Rat CD8 PE |
100T |
| ER0804-50 |
Rat CD8 PE |
50T |
钙离子检测
| 目录号 |
产品 |
规格 |
| F1243 |
fluo-3, AM |
50ug |
同型对照
| 目录号 |
产品 |
规格 |
| M101 |
Mouse IgG1 FITC |
20T |
| M104 |
Mouse IgG1 PE |
20T |
| M105 |
Mouse IgG1 APC |
20T |
| M106 |
Mouse IgG1 PE-Cy5 |
20T |
| M112 |
Mouse IgG1 PE-Cy7 |
20T |
| M118 |
Mouse IgG1 PE-Cy5.5 |
20T |
| M120 |
Mouse IgG1 Alexa Fluor 488 |
20T |
| M2a01 |
Mouse IgG2a FITC |
20T |
| M2a04 |
Mouse IgG2a PE |
20T |
| M2a05 |
Mouse IgG2a APC |
20T |
| M2a06 |
Mouse IgG2a PE-Cy5 |
20T |
| M2b01 |
Mouse IgG2b FITC |
20T |
| M2b04 |
Mouse IgG2b PE |
20T |
| M2b05 |
Mouse IgG2b APC |
20T |
| MM01 |
Mouse IgM FITC |
20T |
| MM04 |
Mouse IgM PE |
20T |
| RG101 |
Rat IgG1 FITC |
20T |
| RG104 |
Rat IgG1 PE |
20T |
| RG105 |
Rat IgG1 APC |
20T |
| RG106 |
Rat IgG1 PE-Cy5 |
20T |
| RG2a01 |
Rat IgG2a FITC |
20T |
| RG2a04 |
Rat IgG2a PE |
20T |
| RG2a05 |
Rat IgG2a APC |
20T |
| RG2a06 |
Rat IgG2a PE-Cy5 |
20T |
| RG2b01 |
Rat IgG2b FITC |
20T |
| RG2b04 |
Rat IgG2b PE |
20T |
| RG2b05 |
Rat IgG2b APC |
20T |
| RG2b06 |
Rat IgG2b PE-Cy5 |
20T |
| H101 |
Hamster IgG FITC |
20T |
| H104 |
Hamster IgG PE |
20T |
荧光二抗
| 目录号 |
产品 |
规格 |
| GAR001 |
Goat Anti-Rabbit IgG(H+L),FITC |
100ul |
| RAG001 |
Rabbit anti-Goat IgG(H+L),FITC |
100ul |
| GAM0041 |
Goat Anti-Mouse IgG (H+L), PE |
100ul |
| GAR0042 |
Goat Anti-Rabbit IgG(γ),PE |
100ul |
| SAG0041 |
Swine Anti-Goat IgG[H+L],PE |
100ul |
| GAM0041 |
Goat Anti-Mouse IgG (H+L), PE |
100ul |
| GAR0042 |
Goat Anti-Rabbit IgG(γ),PE |
100ul |
| SAG0041 |
Swine Anti-Goat IgG[H+L],PE |
100ul |
| GAM6492 |
DyLight649 goat anti-mouse IgG (H+L) |
100ul |
| GAR6492 |
DyLight649 goat anti-rabbit IgG [H+L] |
100ul |
| DAG6492 |
DyLight649 Donkey anti-goat IgG [H+L] |
100ul |
四、样本处理及其他辅助试剂
溶血素（红细胞裂解液）
| 目录号 |
产品 |
规格 |
备注 |
| LSB01 |
Lysing solution for FACS 10X |
100T |
含固定剂，适用于BD流式细胞仪 |
| LSB02 |
Lysing solution for FACS 10X |
250T |
| LSB03 |
Lysing solution for FACS 10X |
500T |
| LSC01 |
FCM Lysing solution for BC(ready-to-use) |
100T |
含固定剂，适用于BeckmanCoulter流式细胞仪 |
| LSC02 |
FCM Lysing solution for BC(ready-to-use) |
250T |
| LYS01 |
FCM Lysing solution(Fixative Free) 10X |
100T |
不含固定剂，适用于染色前溶血或间标流式 |
| LYS02 |
FCM Lysing solution(Fixative Free) 10X |
250T |
| LYS03 |
FCM Lysing solution(Fixative Free) 10X |
500T |
固定破膜剂
| 目录号 |
产品 |
规格 |
| GAS003 |
FIX&PERM Kit |
50T |
| GAS005 |
FIX&PERM Kit |
100T |
| GAS006 |
FIX&PERM Kit |
200T |
特点：破膜后细胞分群保持完；溶血、破膜、染色一步完成，节约成本，操作简便；保持抗体的特异性、灵敏度以及可重复性
流式染色缓冲液
| 目录号 |
产品 |
规格 |
| S1001 |
Flow cytometry Staining buffer |
125ml |
特点：使用方便，即用型；价格便宜；有效降低非特异性结合；适用于各种流式实验
4%多聚甲醛、固定剂
| 目录号 |
产品 |
规格 |
| F0001 |
多聚甲醛溶液，4% |
100mL |
特点：使用方便，即用型；多聚甲醛有毒，配制困难，一般要一天时间。
刺激阻断剂——Th1/Th2/Th17检测必备
高品质原料
• 来自Sigma
使用方便
• 以浓缩液形式提供，无需繁琐计算，按比例加入即可
结合了BFA和Monensin的特性
• 可以将刺激时间延长至18小时，更容易检测
• 方便实验安排，可以过夜培养
| 目录号 |
产品 |
规格 |
| CS0001 |
Phorbol 12-myristate 13-acetate （PMA，佛波酯）0.1mg/ml in Ethanol |
100ul |
| CS0002 |
Ionomycin Calcium（离子霉素）1mg/ml in Ethanol |
50ul |
| CS0003 |
Brefeldin A（BFA）4mg/ml in Ethanol |
60ul |
| CS0004 |
Monensin Sodium（莫能霉素）50mg/ml in Ethanol |
100ul |
| CS1001 |
PMA/Ionomycin mixture(250X) |
100ul |
| CS1002 |
BFA/Monensin Mixture(250X) |
100ul |
| CS1003 |
PMA/Ionomycin/BFA/Monensin mixture(250X) |
100ul |
组织样本制备
| 目录号 |
产品 |
规格 |
| M5205 |
DNase I from bovine pancreas Type IV(10KU/ml) |
200ul |
| M8315 |
Collagenase from Clostridium histolyticum(10mg/ml) |
500ul |
封闭试剂
| 目录号 |
产品 |
规格 |
| A3828 |
Bovine serum Albumin(BSA) |
5g |
| A3828-100 |
Bovine serum Albumin(BSA) |
100g |
| NGS010 |
Normal Goat Serum |
10ml |
| NRS010 |
Normal Rabbit Serum |
10ml |
| NRS500 |
Normal Rat Serum |
500ul |
| EM1632-50 |
anti-mouse CD16/32(Fc Receptor block) |
50T |
| EM1632-100 |
anti-mouse CD16/32(Fc Receptor |
100T |
