Human KLRG1检测金标准

Post published:2016-02-15
Post category:生物资讯
Post views:3134 次访问

https://www.liankebio.com/article-information_Newsletter-1747.html

作者：联科生物发布日期：2016-02-15 13:00

目前已经证实，NK细胞和CD8+T细胞拥有许多共同的抑制性受体，这些抑制性受体在他们的功能和表型特征分析中起着十分重要的作用。KLRG1(killer cell lectin-like receptor G1)，杀伤性细胞凝集素样受体G1,就是其中一种抑制分子。目前研究发现，KLRG1在NK细胞和部分CD8+T细胞，以及一些肥大细胞和嗜碱性粒细胞中有较高水平表达。尽管KLRG1已经作为小鼠 CD8+T细胞衰老和终端效应的分化的关键指标，但是KLRG1在人类细胞中的功能仍不清晰。与小鼠相似，有研究表明，与KLRG1-的细胞相比，KLRG1+CD8+T细胞更易衰老，更弱的增殖能力。

截止目前，市面上还没有商业化试剂能用流式细胞术的方法检测KLRG1表达。但值得高兴的是，现在eBioscience通过弗赖堡大学Dr. Hanspeter Pircher研究出的克隆号为：13F12F2 and 13A2的抗体完美的解决了用流式细胞术的方法检测human KLRG1的标准。

Anti-Human KLRG1.
Staining of normal human peripheral blood cells with Anti-Human CD8a APC (cat. 17-0088)
and Mouse IgG2a K Isotype Control PE (cat. 12-4724) (left) or Anti-Human KLRG1 PE (right).
正常人PBMC中淋巴用于染色分析

产品列表：

Name	Clone	Application	Cat#
Anti-Human KLRG1 PE	13A2	FC	85-12-9487-41
			85-12-9487-42
Anti-Human KLRG1 Alexa Fluor® 488	13F12F2	FC	85-53-9488-41
			85-53-9488-42
Anti-Human KLRG1 APC	13F12F2	FC	85-17-9488-41
			85-17-9488-42
Anti-Human KLRG1 PE	13F12F2	FC	85-12-9488-41
			85-12-9488-42
Anti-Human KLRG1 PerCP-eFluor® 710	13F12F2	FC	85-46-9488-41
			85-46-9488-42
Anti-Mouse KLRG1 APC	2F1	FC	85-17-5893-81
			85-17-5893-82
Anti-Mouse KLRG1 APC-eFluor® 780	2F1	FC	85-47-5893-80
			85-47-5893-82
Anti-Mouse KLRG1 Biotin	2F1	FC	85-13-5893-82
Anti-Mouse KLRG1 eFluor® 450	2F1	FC	85-48-5893-80
			85-48-5893-82
Anti-Mouse KLRG1 FITC	2F1	FC	85-11-5893-80
			85-11-5893-82
Anti-Mouse KLRG1 Functional Grade Purified	2F1	FC, FA	85-16-5893-81
			85-16-5893-82
			85-16-5893-85
Anti-Mouse KLRG1 PE	2F1	FC	85-12-5893-80
			85-12-5893-82
Anti-Mouse KLRG1 PE-Cyanine7	2F1	FC	85-25-5893-80
			85-25-5893-82
Anti-Mouse KLRG1 PE-eFluor® 610	2F1	FC	85-61-5893-80
			85-61-5893-82
Anti-Mouse KLRG1 PerCP-eFluor® 710	2F1	FC	85-46-5893-80
			85-46-5893-82
Anti-Mouse KLRG1 Purified	2F1	FC, IP	85-14-5893-81
			85-14-5893-82
			85-14-5893-85

【参考文献】
Schwartzkopff S., et al. TGF-β downregulates KLRG1 expression in mouse and human CD8(+) T cells. European Journal ofImmunology 45(8):2212–2217 (2015).

Dolfi D.V., et al. Increased T-bet is associated with senescence of influenza virus-specific CD8 T cells in aged humans. Journal ofLeukocyte Biology 93(6):825–836 (2013).

Voehringer D., et al. Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1(KLRG1). Blood 100(10):3698–3702 (2002).

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