GAR007

Goat Anti-Rabbit IgG(H+L) HRP 山羊抗兔二抗-HRP

¥289.00¥350.00

在售SKU:70-GAR0072, 70-GAR007, Goat, Rabbit, IgG, HRP, 山羊, 二抗, IgG(H+L), 山羊抗兔, 羊抗兔, 二抗-HRP


描述

背景简介

第二抗体是能和抗体结合,即抗体的抗体,其主要作用是检测抗体的存在,放大一抗的信号。二抗是利用抗体是大分子的蛋白质具有抗原性的性质,去免疫异种动物,由异种动物的免疫系统产生的针对于此抗体的免疫球蛋白。

产品特点

  • 性价比高,推荐稀释比例:1:500—1:5000(IHC)、1:5000—1:100000(WB/ELISA)
  • 无需分装不冻融
  • 价格便宜,常备现货

如何选择二抗

通常情况下,某一实验中可能同时有几种二抗可供选择,如何能选择到最适合该实验的二抗,需要综合以下几个方面进行考虑:

一、 一抗的种属来源

一抗是什么物种来源的,相应的二抗也要是抗该物种的抗体。

二、 一抗的免疫球蛋白亚型

二抗需与一抗的类别或亚型相匹配。例如一抗是IgM,则须选择抗IgM的二抗。如果一抗是某一亚型(如IgG1、IgG2a、IgG2b、IgG3、IgG4等),则可选择能与所有亚型结合的抗IgG二抗,也可选择专门针对该亚型的二抗。

三、 实验方案

根据实验,选择酶标记(如HRP)的二抗,还是荧光素标记的二抗。根据仪器的激发光和检测通道,选择合适的荧光素。

四、 选择哪种形式的二抗,是整个分子还是F[ab’]2片段?

  • 整个IgG分子:此种抗体适用于多数情况。
  • F[ab’]2片段:该抗体由 2 个二硫键连接的 Fab 片段组成,用于特定的情况中,如需要避免抗体与具有Fc 受体的细胞结合,降低非特异性背景。

引用文献

  1. Effects of Lentivirus-Mediated Endostatin on Endothelial Progenitor Cells 

  2. Involvement of Viperin in Prevention of Intrauterine Transmission of Hepatitis B Virus 

  3. Tnf-Α Promotes Survival and Migration of Mscs under Oxidative Stress Via Nf-Κb Pathway to Attenuate Intimal Hyperplasia in Vein Grafts 

  4. Expression of B Cell-Specific Moloney Murine Leukemia Virus Integration Site 1 in Vulvar Squamous Cell Carcinoma and Its Effect on the Biological Behavior of a-431 Cells 

  5. TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss 

  6. Alantolactone-loaded chitosan/hyaluronic acid nanoparticles suppress psoriasis by deactivating STAT3 pathway and restricting immune cell recruitment 

  7. An Immune-Induced Reeler Protein Is Involved in the Bombyx Mori Melanization Cascade 

  8. Autophagy-Regulated Ampar Subunit Upregulation in in Vitro Oxygen Glucose Deprivation/Reoxygenation-Induced Hippocampal Injury 

  9. Reduced Expression of Sushi Domain Containing 2 Is Associated with Progression of Non-Small Cell Lung Cancer 

  10. Inhibition of Farnesyl Pyrophosphate Synthase Attenuates High Glucose?Induced Vascular Smooth Muscle Cells Proliferation 

  11. Tissue-Specific Effects of Atorvastatin on 3-Hydroxy-3-Methylglutarylcoenzyme a Reductase Expression and Activity in Spontaneously Hypertensive Rats 

  12. The Regenerating Gene Iα Is Overexpressed in Atrophic Gastritis Rats with Hypergastrinemia 

  13. Reversing Multidrug Resistance in Hepatocellular Carcinoma Cells by Inhibiting Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase Signaling Pathway Activity 

  14. A Vps21 Endocytic Module Regulates Autophagy 

  15. The Role of Caveolin-1 in Blood-Brain Barrier Disruption Induced by Focused Ultrasound Combined with Microbubbles 

  16. Neurogenin 2 Enhances the Neuronal Differentiation of Skin-Derived Precursors 

  17. Protective Effect of Momordica Charantia Water Extract against Liver Injury in Restraint-Stressed Mice and the Underlying Mechanism 

  18. Inhibition of Farnesyl Pyrophosphate Synthase Attenuates Angiotensin Ii-Induced Fibrotic Responses in Vascular Smooth Muscle Cells 

  19. Low Expression of Pinx1 Is Associated with Malignant Behavior in Basal-Like Breast Cancer 

  20. Transforming Growth Factor-Beta3 Expression up-Regulates on Cleft Palates Induced by 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in Mice 

  21. Alcohol Exposure Increases the Expression of Cardiac Transcription Factors through Erk1/2-Mediated Histone3 Hyperacetylation in H9c2 Cells 

  22. Synergistic Antitumor Effects of Cdk Inhibitor Sns?032 and an Oncolytic Adenovirus Co?Expressing Trail and Smac in Pancreatic Cancer 

  23. Curcumin Suppresses Cell Proliferation through Inhibition of the Wnt/Β-Catenin Signaling Pathway in Medulloblastoma 

  24. Cd36 Mediated Fatty Acid-Induced Podocyte Apoptosis Via Oxidative Stress 

  25. Flavopiridol Protects Bone Tissue by Attenuating Rankl Induced Osteoclast Formation 

  26. Splicing Factor Hnrnpa2b1 Contributes to Tumorigenic Potential of Breast Cancer Cells through Stat3 and Erk1/2 Signaling Pathway 

  27. Aberrant Expression of the Autocrine Motility Factor Receptor Correlates with Poor Prognosis and Promotes Metastasis in Gastric Carcinoma 

  28. Development of a Capture Elisa to Determine Kinetics of Soluble Cd25 Following in Vitro and in Vivo Stimulation of Duck Peripheral Blood Monocytes 

  29. Microrna-28 Potentially Regulates the Photoreceptor Lineage Commitment of Müller Glia-Derived Progenitors 

  30. Implantation of Bone Mesenchymal Stem Cells Overexpressing Mirna?705 Mitigated Ischemic Brain Injury 

  31. Next Generation Sequencing Analysis of Mirnas: Mir-127-3p Inhibits Glioblastoma Proliferation and Activates Tgf-Β Signaling by Targeting Ski 

  32. Autophagy Protects against Palmitic Acid-Induced Apoptosis in Podocytes in Vitro 

  33. The Antifibrotic Effects of Tgf-Β1 Sirna on Hepatic Fibrosis in Rats 

  34. Development and Validation of a Three-Gene Prognostic Signature for Patients with Hepatocellular Carcinoma 

  35. Revealing Chemical Processes and Kinetics of Drug Action within Single Living Cells Via Plasmonic Raman Probes 

  36. Aggravation of Acute Kidney Injury by Mpges-2 Down Regulation Is Associated with Autophagy Inhibition and Enhanced Apoptosis 

  37. Resveratrol Ameliorates Oxidative Stress and Inhibits Aquaporin 4 Expression Following Rat Cerebral Ischemia-Reperfusion Injury 

  38. RAW 264.7

    Oxidized Low-Density Lipoprotein Activates Adipophilin through Erk1/2 Signal Pathway in Raw264.7 Cells 

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  40. Acute Hyperglycemia Together with Hematoma of High-Glucose Blood Exacerbates Neurological Injury in a Rat Model of Intracerebral Hemorrhage 

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  45. Activating Autophagy in Hippocampal Cells Alleviates the Morphine-Induced Memory Impairment 

  46. Fusion Peptides Cpu1 and Cpu2 Inhibit Matrix Metalloproteinases and Protect Mice from Endotoxin Shock within a Strict Time Window 

  47. Inhibition of Neutrophil Collagenase/Mmp-8 and Gelatinase B/Mmp-9 and Protection against Endotoxin Shock 

  48. Effects of Exosome-Mediated Delivery of Myostatin Propeptide on Functional Recovery of Mdx Mice 

  49. Effects of Gastrokine?2 Expression on Gastric Cancer Cell Apoptosis by Activation of Extrinsic Apoptotic Pathways 

  50. Ectopic Expression of Hoxb4a in Hemangioblasts Promotes Hematopoietic Development in Early Embryogenesis of Zebrafish 

  51. Additive Antiangiogenesis Effect of Ginsenoside Rg3 with Low-Dose Metronomic Temozolomide on Rat Glioma Cells Both in Vivo and in Vitro 

  52. Microrna-10b Induces Glioma Cell Invasion by Modulating Mmp-14 and Upar Expression Via Hoxd10 

  53. Insulin Restores Ucp3 Activity and Decreases Energy Surfeit to Alleviate Lipotoxicity in Skeletal Muscle 

  54. Mechanism of Inactivation of Murine Norovirus-1 by High Pressure Processing 

  55. Integrated Analysis of Microrna Regulatory Network in Nasopharyngeal Carcinoma with Deep Sequencing 

  56. Phloridzin Improves Lipoprotein Lipase Activity in Stress-Loaded Mice Via Ampk Phosphorylation 

  57. Epidermal Growth Factor Receptor-Regulated Mir-125a-5p--a Metastatic Inhibitor of Lung Cancer 

  58. Pkcα Inhibited Apoptosis by Decreasing the Activity of Jnk in Mcf-7/Adr Cells 

  59. Clozapine-Induced Reduction of L-Carnitine Reabsorption Via Inhibition/Down-Regulation of Renal Carnitine/Organic Cation Transporter 2 Contributes to Liver Lipid Metabolic Disorder in Mice 

  60. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced by Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway 

  61. Expression of Irak?3 Is Associated with Colitis?Associated Tumorigenesis in Mice 

  62. Expression and Immunogenicity of Novel Subunit Enterovirus 71 Vp1 Antigens 

  63. Rosuvastatin Prevents Pressure Overload?Induced Myocardial Hypertrophy Via Inactivation of the Akt, Erk1/2 and Gata4 Signaling Pathways in Rats 

  64. P2y6 Receptor-Mediated Microglial Phagocytosis in Radiation-Induced Brain Injury 

  65. Inhibition of Phosphodiesterase-4 Reverses Aβ-Induced Memory Impairment by Regulation of Hpa Axis Related Camp Signaling 

  66. An Autophagosome-Based Therapeutic Vaccine for Hbv Infection: A Preclinical Evaluation 

  67. Skeletal Muscle Aldolase an Overexpression in Endotoxemic Rats and Inhibited by Gsno Via Potential Role for S-Nitrosylation in Vitro 

  68. Enhanced Aerobic Glycolysis by S-Nitrosoglutathione Via Hif-1α Associated Glut1/Aldolase a Axis in Human Endothelial Cells 

  69. Zoledronate Attenuates Angiogenic Effects of Angiotensin Ii-Stimulated Endothelial Progenitor Cells Via Rhoa and Mapk Signaling 

  70. Does the Bone Morphogenetic Protein 7 Inhibit Oocyte Maturation by Autocrine/Paracrine in Mud Crab? 

  71. Vitellogenin2: Spermatozoon Specificity and Immunoprotection in Mud Crabs 

  72. Microrna-141 Inhibits Tumor Growth and Minimizes Therapy Resistance in Colorectal Cancer 

  73. Pulmonary C-Fiber Degeneration Downregulates Ifn-Γ Receptor 1 Via Ifn-Α Induction to Attenuate Rsv-Induced Airway Hyperresponsiveness 

  74. Neuroprotective Effect of Curcumin against Oxidative Damage in Bv-2 Microglia and High Intraocular Pressure Animal Model 

  75. Expression of Potential Cancer Stem Cell Marker Abcg2 Is Associated with Malignant Behaviors of Hepatocellular Carcinoma 

  76. Involvement of Exogenous 3?Deoxyglucosone in Β?Cell Dysfunction Induces Impaired Glucose Regulation 

  77. High Expression of Tmem40 Contributes to Progressive Features of Tongue Squamous Cell Carcinoma 

  78. Thiazolidinediones Improve Hepatic Fibrosis in Rats with Non-Alcoholic Steatohepatitis by Activating the Adenosine Monophosphate-Activated Protein Kinase Signalling Pathway 

  79. Enhanced Expression of Markers for Astrocytes in the Brain of a Line of Gfap-Tk Transgenic Mice 

  80. Dnaj of Streptococcus Suis Type 2 Contributes to Cell Adhesion and Thermotolerance 

  81. Down-Regulation of Nuclear Hmgb1 Reduces Ischemia-Induced Hmgb1 Translocation and Release and Protects against Liver Ischemia-Reperfusion Injury 

  82. Mycobacterial 3-Hydroxyacyl-L-Thioester Dehydratase Y Derived from Mycobacterium Tuberculosis Induces Cox-2 Expression in Mouse Macrophages through Mapk-Nf-Κb Pathway 

  83. Cytotoxic Effect and Apoptotic Mechanism of Tanshinone a, a Novel Tanshinone Derivative, on Human Erythroleukemic K562 Cells 

  84. Effects of Matrix Metalloproteinase-1 on the Myogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Vitro 

  85. Selective Preservation of Cholinergic Mecp2 Rescues Specific Rett-Syndrome-Like Phenotypes in Mecp2(Stop) Mice 

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  87. Involvement of Spinal Sirt1 in Development of Chronic Constriction Injury Induced Neuropathic Pain in Rats 

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