人α干扰素高敏 ELISA 试剂盒Human IFN-α High Sensitivity ELISA Kit

人干扰素(IFNs) 高敏ELISA试剂盒Human IFN-α High Sensitivity ELISA Kit

本试剂盒采用双抗体夹心酶联免疫吸附检测技术。

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品牌：	MultiSciences	目录号：	70-EK199HS-96
规格：	96 T	应用：	ELISA
反应性：	Human	标记：	NoData

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品牌：MultiSciences
所属分类：ELISA试剂盒

【背景介绍】干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的 一组信号蛋白。在通常情况下，病毒感染的细胞会释放干扰素，使周围的细胞提高其抗病毒防御 能力。基于受体类型，人类干扰素可分为 3 大类型：I 型干扰素，包括 IFN-α、IFN-β、IFN-ε、IFN-κ 和 IFN-ω，II 型干扰素(在人类中称为 IFN-γ)和 III 型干扰素。IFN-α 蛋白由白细胞产生，主要参与响应病毒感染的先天性免疫。研究显示加强肿瘤浸润巨噬 细胞中 IFN-α 的表达可引起更有效的树突状细胞活化和免疫效应细胞毒作用。IFN-α 作为致热因子， 可通过改变下丘脑中热敏神经元的活性来导致发热。运用类似机理，IFN-α 可用来减轻疼痛，与 μ- 阿片受体相互作用来作为止痛剂。【DESCREPTION】Interferons (IFNs) are a group of signaling proteins made and released by host cells in response to the presence of pathogens, such as viruses, bacteria, parasites, or tumor cells. In a typical scenario, a virus-infected cell will release interferons causing nearby cells to heighten their anti-viral defenses. Based on the type of receptor, human interferons have been classified into three major types: interferon type I, including IFN-α, IFN-β, IFN-ε, IFN-κ and IFN-ω, interferon type II (IFN-γ in humans) and interferon type III.The IFN-α proteins are produced by leukocytes. They are mainly involved in innate immune response against viral infection. Studies suggest that by forcing IFN-α expression in tumor-infiltrating macrophages, it is possible to elicit a more effective dendritic cell activation and immune effector cell cytotoxicity. IFN-α acts as a pyrogenic factor by altering the activity of thermosensitive neurons in the hypothalamus thus causing fever. A similar mechanism is used by IFN-α to reduce pain. IFN-α interacts with the μ-opioid receptor to act as an analgesic.【检测原理】本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 IFN-α 单克隆抗体预包被在高 亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的 IFN-α 与固相 抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素 化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂 孵育，洗涤去除未结合的物质后，再次加入 streptavidin-HRP。洗涤后，加入显色底物 TMB，避光 显色。颜色反应的深浅与样本中 IFN-α 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参 考波长 570 - 630 nm)测定吸光度值。【PRINCIPLE OF THE ASSAY】This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IFN-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IFN-α present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IFN-α is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IFN-α bound in the initial step. The color development is stopped and the intensity of the color is measured. 【分析类型】夹心ELISA【样本类型】血清、血浆、细胞上清【板式】96孔板，可拆【保存】试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。【运输条件】4℃【样本体积】20 μl【灵敏度】0.23 pg/ml <img src="http://120.27.244.130/public/upload/goods/Standard-EK199HS.jpg" /> 【标准曲线范围】7.81 - 500 pg/ml【回收率范围】95 %-119 %【平均回收率】1.05【板间变异系数】4.1 % - 5.0 %【板内变异系数】3.9 % - 4.9 %【上市时间】20190329

人干扰素(IFNs) 高敏ELISA试剂盒Human IFN-α High Sensitivity ELISA Kit

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