Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting.However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example,expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Full-length β-Tubulin protein of human
1mg/ml in PBS, pH 7.4, containing 0.1% proclin and 50% glycerol
Fig: Western blot analysis of Hela cell lysate (lane1) , rat brain tissue lysate (lane2) and mouse brain tissue lysate (lane3) with β-Tubulin mouse mAb (3G7) diluted at 1:5000.
Fig: Western blot analysis of Hela cell lysate with β-Tubulin mouse mAb (3G7) diluted at 1:5000 (lane1), 1:10000
(lane2), 1:20000 (lane3) and 1:40000 (lane4).
Fig: Immunohistochemistry Staining of human squamous cell carcinoma of cervical and prostate cancer with β-tubulin mouse mAb (3G7)
Fig: Immunofluorescence Staining of SW480 cell with
β-Tubulin mouse mAb(3G7).
Fig.1: Nuclei stained with DAPI
Fig.2: ImmunoFluorescent analysis with β-Tubulin
Fig.3: Merge (1+2).