EK180

Human IFN-gamma ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

  • 分子靶点:IFNG, IFN-γ
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:50 μL;细胞培养上清:100 μL
  • 灵敏度:0.30 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:83% - 113%

在售SKU:70-EK180-48, 70-EK180-96


描述

商品名

Human IFN-γ ELISA Kit (人γ干扰素ELISA 试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.6% - 4.5%;板间变异系数:4.5% - 7.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:50 μL;细胞培养上清:100 μL

灵敏度

0.30 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

83% - 113%

平均回收率

0.95

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗人IFN-γ单克隆抗体的96孔聚苯乙烯酶标板
  • 人IFN-γ冻干标准品
  • IFN-γ 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IFN-γ ELISA Kit (人γ干扰素ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名:IFNG, interferon gamma
  • 基因家族:Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。

人 Human IFNG 分子靶点信息

  • 分子名:IFNG, interferon gamma
  • 别称:
    • IFG
    • IFI
    • IFN-gamma
    • IMD69
    • immune interferon
    • interferon, gamma
  • 基因序列:NCBI_Gene: 3458
  • 蛋白序列:UniProtKB: P01579

人 Human IFNG靶点分子功能(预测)

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

操作步骤


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引用文献

  1. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  2. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  3. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  4. 细胞培养上清

    Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 

  5. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  6. 血清

    Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

  7. 细胞培养上清 血清

    Casein Kinase II exacerbates rheumatoid arthritis via promoting Th1 and Th17 cell inflammatory responses 

  8. 细胞培养上清

    PDL1-targeted vaccine exhibits potent antitumor activity by simultaneously blocking PD1/PDL1 pathway and activating PDL1-specific immune responses 

  9. Association Analysis of Polymorphisms in Oas1 with Susceptibility and Severity of Hand, Foot and Mouth Disease 

  10. Inverse Relationship between Toxic Shock Syndrome Toxin-1 Antibodies and Interferon-Γ and Interleukin-6 in Peripheral Blood Mononuclear Cells from Patients with Pediatric Tonsillitis Caused by Staphylococcus Aureus 

  11. Development of T Cells Redirected to Glypican-3 for the Treatment of Hepatocellular Carcinoma 

  12. Aryl Hydrocarbon Receptor Modulates the Expression of Tnf-Α and Il-8 in Human Sebocytes Via the Myd88-P65nf-Κb/P38mapk Signaling Pathways 

  13. A Multistage Subunit Vaccine Effectively Protects Mice against Primary Progressive Tuberculosis, Latency and Reactivation 

  14. Accurate Control of Dual-Receptor-Engineered T Cell Activity through a Bifunctional Anti-Angiogenic Peptide 

  15. Antigen Presentation of the Oct4 and Sox2 Peptides by Cd154-Activated B Lymphocytes Enhances the Killing Effect of Cytotoxic T Lymphocytes on Tumor Stem-Like Cells Derived from Cisplatin-Resistant Lung Cancer Cells 

  16. Microrna-34a Promotes Micb Expression in Hepatocytes 

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