https://www.liankebio.com/article-information_Newsletter-417.html
作者：联科生物 发布日期：2016-02-16 00:00
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T细胞要达到完全活化，至少需要两个信号:第一信号是由TCR识别抗原-主要组织相容性抗原MHC所启动；第二信号是由T细胞与协同共刺激分子相互作用所启动。而T细胞最重要的共刺激分子是CD28. 这些信号被传送到细胞核内后，会引起：
1.T细胞的克隆性增殖；
2.细胞表面活化标记物的上调
3.效应细胞的分化
4.细胞毒性诱导或细胞因子分泌
5.凋亡诱导反应
T细胞活化示意图
体外T细胞活化方案步骤：
eBio：http://media.ebioscience.com/data/pdf/best-protocols/t-cell-activation-in-vitro.pdf
产品列表：
| Name
|
Clone
|
Application
|
Cat#
|
| Anti-Mouse CD3e Functional Grade Purified 50 ug
|
145-2C11
|
FC, FA
|
85-16-0031-81
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| Anti-Mouse CD3e Functional Grade Purified 100 ug
|
85-16-0031-82
|
| Anti-Mouse CD3e Functional Grade Purified 500 ug
|
85-16-0031-85
|
| Anti-Mouse CD3e Functional Grade Purified 1 mg
|
85-16-0031-86
|
| Anti-Mouse CD28 Functional Grade Purified 50 ug
|
37.51
|
FC, FA
|
85-16-0281-81
|
| Anti-Mouse CD28 Functional Grade Purified 100 ug
|
85-16-0281-82
|
| Anti-Mouse CD28 Functional Grade Purified 500 ug
|
85-16-0281-85
|
| Anti-Mouse CD28 Functional Grade Purified 1 mg
|
85-16-0281-86
|
| Anti-Human CD3 Functional Grade Purified 50 ug
|
OKT3
|
FC, FA
|
85-16-0037-81
|
| Anti-Human CD3 Functional Grade Purified 500 ug
|
85-16-0037-85
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| Anti-Human CD3 Functional Grade Purified 50 ug
|
HIT3a
|
FC, FA
|
85-16-0039-81
|
| Anti-Human CD3 Functional Grade Purified 500 ug
|
85-16-0039-85
|
| Anti-Human CD28 Functional Grade Purified 50 ug
|
CD28.2
|
FC, FA
|
85-16-0289-81
|
| Anti-Human CD28 Functional Grade Purified 500 ug
|
85-16-0289-85
|
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