Protein A是从A型金黄色葡萄球菌(Staphylococcus aureus)分离而得的一种细胞壁蛋白。而Prote in G则是从G型链球菌分离而得的细胞壁蛋白。两者均可与免疫球蛋白的非抗原结合点结合， 但其结合能力与免疫球蛋白的种属和亚型相关，具体参见右边表格。Protein A和ProteinG 在亲和力、稳定性方面好，可替代二抗，广泛应用于免疫学领域。
| Ig来源 | Protein A | Protein G |
| Human IgG1,2,4 | Strong | Strong |
| Human IgG3 | No binding | Strong |
| Human IgA, D, E | Weak | No binding |
| Mouse IgG1 | Weak | Strong |
| Mouse IgG2a, 2b, 3 | Strong | Strong |
| Mouse IgM | Weak | No binding |
| Rat IgG1 | Weak | Weak |
| Rat IgG2a | No binding | Strong |
| Rat IgG2b | No binding | Weak |
| Rat IgG2c | Strong | Strong |
| Bovine IgG1 | Weak | Strong |
| Bovine | Strong | Strong |
| Chicken IgY | No binding | No binding |
| Dog IgG | Strong | Weak |
| Goat IgG1 | Weak | Strong |
| Goat IgG2 | Strong | Strong |
| Guinea pig IgG | Strong | Weak |
| Hamster | Weak | - |
| Horse IgG | Weak | Strong |
| Monkey IgG | Strong | Strong |
| Porcine IgG | Strong | Strong |
| Rabbit IgG | Strong | Strong |
| Sheep IgG1 | Weak | Strong |
| Sheep IgG2 | Strong | Strong |
Rockland提供多种标记的Protein A和Protein G产品，可用于ELISA、FLISA、IF、IHC、WB等实验。
| 产品名称 | 货号 | 规格 | 应用 | 目录价(￥) |
| Protein A | PA00-00 | 5mg | ELSIA, WB | 1600.00 |
| Protein A Biotin Conjugated | PA00-06 | 500μg | ELSIA, WB, IHC | 880.00 |
| Protein A DyLight™ 488 Conjugated | PA00-41 | 100μg | FLSIA, WB, IF | 1280.00 |
| Protein A IRDye700Dx® Conjugated | PA00-30 | 500μl | FLSIA, WB | 2080.00 |
| Protein G | PG00-00 | 1mg | ELSIA, WB | 2160.00 |
| Protein G Fluorescein Conjugated | PG00-02 | 1mg | FLSIA, IF | 2240.00 |
| Protein G Peroxidase Conjugated | PG00-03 | 1mg | ELSIA, WB, IHC | 2400.00 |
| Protein G IRDye800® Conjugated | PG00-032 | 500μl | FLSIA, WB | 2080.00 |
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